For Research Use Only. Not for use in diagnostic procedures. Others Ribo CC Plus RUO Ribo CC Plus PID00000160 05470331001 DISC. Ribo CC+ RUO DISCOVERY Ribo CC 04015630976980 Reagents, kits 760-213 1 x 1L bottle Not Available undefined For Research Use Only. Not for use in diagnostic procedures.VENTANA Ribo CC Plus is a prediluted solution used as a pretreatment step in the processing of tissue samples for immunohistochemistry (IHC) and in situ hybridization (ISH) reactions carried out on VENTANA DISCOVERY Series automated slide stainers (DISCOVERY, DISCOVERY XT, and DISCOVERY ULTRA). en Fixation of tissue by formalin and formaldehyde results in the formation of covalent bonds between the aldehyde and amino groups present in the tissue. The formation of these bonds denatures protein and can result in the loss of antigenicity. In addition, the formaldehyde can form methylene bridges, which cross link tissue proteins, thus reducing the penetration of the tissue to large molecules such as antibodies.Ribo CC Plus is a citrate buffer at a slightly acidic pH, which, at elevated temperatures, is capable of hydrolyzing the covalent bonds formed by formalin and formaldehyde in tissue. Removing these bonds allows renaturation of protein molecules and increases antibody accessibility. Often these changes result in significant gains in antibody binding and improved signal to noise ratios. The automated slide stainer heats the slide to the appropriate temperature and maintains the temperature for a specific time interval selected by the user. en