For in vitro diagnostic use. Others VENTANA U6 DIG Pro IVD VENTANA® U6 DIG Probe PID00000974 10 062 746 001 10062746001 VENTANA U6 DIG Probe VENTANA U6 DIG Probe 07613336226568 Reagents, kits 760-7237 30 tests Not Available true The VENTANA U6 DIG Probe is optimally formulated for use with the VENTANA Magenta ISH DIG Detection Kit, the ISH TSA Ancillary Kit, and accessory reagents on BenchMark IHC/ISH instruments. During the ISH staining process, DIG-labeled probe is hybridized to complimentary U6 snRNA in the tissue. Probe is detected with the VENTANA Magenta ISH DIG Detection Kit. This detection system uses HRP-labeled anti-DIG mouse monoclonal antibody and DIG-labeled tyramide amplification to visualize the target as a magenta signal through the covalent linkage of sulforhodamine B-labeled tyramide to the tissue (see Figure 2). Figure 2. VENTANA Magenta ISH DIG Detection Kit Refer to the method sheet of this detection kit for additional information. The staining protocol consists of numerous steps in which reagents are incubated for pre- determined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instruments washes the sections to remove unbound material and applies a liquid coverslip that minimizes the evaporation of the aqueous reagents from the slide. Results are visualized and interpreted using a light microscope. Refer to the VENTANA Magenta ISH DIG Detection Kit method sheet for detailed descriptions of: Principles of the Procedure, Materials and Methods, Specimen Collection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Results, and Limitations. en The VENTANA U6 DIG Probe is intended for laboratory use in the qualitative assessment of RNA integrity in sections of formalin-fixed, paraffin- embedded tissue that are stained on a BenchMark IHC/ISH instrument. Staining results should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. The reagent is intended for in vitro diagnostic (IVD) use. en

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