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Carry out RNA-seq library preparation with efficiency and confidence
Overcome your most challenging samples with our proven and complete workflows, designed to handle highly degraded FFPE RNA and precious low-input samples. The KAPA RNA HyperPrep Kits leverage enzymes from our Directed Evolution Technology to maximize RNA conversion, delivering minimal GC bias and exceptionally even coverage for more reliable quantification.
- Unlock reliable data from low-quality or low-input RNA
- Maximize speed and simplicity with a single-day, single-tube workflow
- Tailor the workflows to your needs, without compromise
RNA sequencing (RNA-seq) uses next-generation sequencing (NGS) to comprehensively profile the transcriptome. This powerful technique reveals differentially expressed genes and their abundance, and facilitates the discovery of genetic variant splicing, novel transcripts, gene fusions, isoforms, and single-nucleotide variants. Depending on the selected workflow, results can include both coding and noncoding RNA, only mature polyA transcripts, or only selected RNAs.
During RNA-seq library preparation, RNA transcripts are first converted to complementary DNA (cDNA), with strand-specific methods enhancing accuracy for antisense and non-coding RNA detection, and for defining gene boundaries. RNA library prep can also be coupled with methods to selectively enrich for or deplete certain transcripts. RNA-seq thus offers critical insights into cellular behavior and responses across various biological states.
Featured products
RUO
For Research Use Only. Not for use in diagnostic procedures.
KAPA RNA EvoPrep Kits
Maximize Every Read. Our new KAPA RNA EvoPrep Kit shortens workflow, boosts sequencing efficiency, captures more unique transcripts, and accelerates discovery.
RUO
For Research Use Only. Not for use in diagnostic procedures.
KAPA RNA EvoPrep Kit with RiboErase (HMR)
The KAPA RNA EvoPrep Kit with RiboErase (HMR or Human/Mouse/Rat) contains all of the buffers and enzymes required for depletion of ribosomal RNA (rRNA) and the rapid construction of stranded RNA-Seq libraries from 5 ng – 1 μg of purified total RNA. The KAPA RNA EvoPrep Kit with RiboErase (HMR) is designed for both manual and automated NGS library construction. The kit depletes both cytoplasmic (5S, 5.8S, 18S, and 28S), and mitochondrial (12S and 16S) rRNA species.This workflow is compatible with both high- and low-quality (e.g., extracted from FFPE tissue) RNA.
The protocol is applicable to a wide range of RNA-Seq applications, including:
gene expression analysis;
single nucleotide variation (SNV) discovery;
splice junction and gene fusion identification; and
characterization of both polyadenylated and non-polyadenylated RNAs, including noncoding and immature RNAs.
This kit has not been tested with small RNAs < 100 bp in lenght.
RUO
For Research Use Only. Not for use in diagnostic procedures.
KAPA RNA EvoPrep Kit with RiboErase (HMR) Globin
The KAPA RNA EvoPrep Kit with RiboErase (HMR or Human/Mouse/Rat) Globin contains all of the buffers and enzymes required for depletion of ribosomal RNA (rRNA) and globin mRNA transcripts, and the rapid construction of stranded RNA-seq libraries from 5 ng – 1 μg of blood-derived, purified total RNA.
The KAPA RNA EvoPrep Kit with RiboErase (HMR) Globin is designed for both manual and automated NGS library construction.
The kit depletes both cytoplasmic (5S, 5.8S, 18S, and 28S), and mitochondrial (12S and 16S) rRNA species as well as globin mRNA transcripts.
The protocol is applicable to a wide range of RNA-Seq applications, including:
gene expression analysis;
single nucleotide variation (SNV) discovery;
splice junction and gene fusion identification; and
characterization of both polyadenylated and non-polyadenylated RNAs, including noncoding and immature RNAs.
This kit has not been tested with small RNAs < 100 bp in length.
Benefits of RNA library prep solutions from Roche
Broad applications
Unlock reliable data from low-quality or low-input RNA
RNA sequencing (RNA-seq) offers a powerful lens into the dynamic transcriptome, revealing the intricate details of gene expression and regulation that define cellular states in health and disease.1 However, constructing an effective library from total RNA requires a strategic approach, as samples are typically dominated by abundant species like ribosomal RNA (rRNA) that can obscure detection of more relevant transcripts.
KAPA RNA library prep kits can help you achieve high-quality, reproducible data from your most challenging samples. Our solutions are engineered to handle a wide spectrum of sample types, accounting for various input qualities and amounts to empower your diverse research goals in these three key areas:
- Whole transcriptome sequencing: Measure global expression levels of all coding and non-coding transcripts, identifying exons, introns, and their junctions.
- mRNA capture: Focus on mRNA transcripts to uncover the molecular basis of phenotypic differences and quantify coding gene expression levels.
- RNA depletion: Improve sensitivity and cost-efficiency by removing abundant RNAs (like rRNA or globin). This allows for better detection of rare transcripts, subtle gene expression changes, or achieving experimental goals with less sequencing.
Single-day workflow
Maximize speed and simplicity with a single-day, single-tube workflow
Accelerate RNA-seq library preparation from sample to insight with an efficient workflow. By integrating novel chemistry with optimized enzymatic reactions, this solution dramatically reduces protocol time and complexity without sacrificing data quality.
- Go from RNA to a strand-specific library in as little as four hours
- Reduce protocol time and complexity with fewer purification steps
- Streamline your workflow, from RNA enrichment to an Illumina-ready library in a single day
Robust performance
Tailor the workflows to your needs, without compromise
Achieve superior performance and reliability, even when working with difficult, low-quantity material or degraded RNA from FFPE tissue. Deliver high-quality results with a workflow optimized for exceptional sensitivity and robustness.
- Turn challenging low-input samples into high-quality RNA-seq libraries2
- Succeed with your most difficult samples—from diverse types to degraded FFPETRNA—with a robust workflow1
KAPA RNA library prep kits comparison overview
KAPA RNA EvoPrep Kit
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KAPA RNA EvoPrep Kit with RiboErase (HMR)
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KAPA RNA EvoPrep Kit with RiboErase (HMR) Globin
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KAPA mRNA EvoPrep Kit
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| Enrichment | |||
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No enrichment
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Ribodepletion
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Globin depletion
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mRNA capture
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| Sample type | |||
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Recommended: High-quality total RNA, Poly(A)-selected and/or depleted RNA. Compatible: Low-quality total RNA (e.g., FFPET RNA).
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Recommended: High-quality total RNA. Compatible: Low-quality total RNA.
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Recommended: High-quality total RNA. Compatible: Low-quality total RNA.
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Recommended: High-quality total RNA.
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| Sample input range | |||
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0.25 ng - 100 ng
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5 ng - 1 µg
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5 ng - 1 µg
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25 ng - 1 µg
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| Species | |||
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Eukaryotic, microbe, plant, etc.
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Human, mouse, and rat
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Human, mouse, and rat
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Eukaryotic, microbe, plant, etc.
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| Fragmentation | |||
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Tunable
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Tunable
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Tunable
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Tunable
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| Library prep time | |||
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3.5 hrs
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5.5 hrs
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5.5 hrs
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4.5 hrs
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| Automation friendly | |||
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Yes
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Yes
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Yes
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Yes
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*Custom depletion protocol support available for other organisms or transcripts
Automation-friendly NGS library preparation
Thinking of automating library preparation? Roche provides NGS automation support with a menu of automated methods for KAPA library prep reagents on non-Roche liquid handling vendors, and on Roche's AVENIO Edge System.
KAPA products are for Research Use Only. Not for use in diagnostic procedures
The AVENIO Edge System is a Class 1 US IVD and a Class A CE IVD (under IVDR) and is intended for downstream diagnostic applications. For the EU, Roche is collaborating with Platomics to enable laboratories to help automate document generation on Platomics’ multi-stakeholder platform for workflows incorporating the Avenio Edge System. Reagents and kits mentioned are for Research Use Only. They are not intended for diagnostic procedures. The AVENIO Edge System can be used for Research Use Only (RUO) workflows.
References
- Smail C and Montgomery SB. RNA sequencing in disease diagnosis. Annual Review of Genomics and Human Genetics. 2024;25:353–367. Available from: https://www.annualreviews.org/content/journals/10.1146/annurev-genom-021623-121812
- F. Hoffmann-La Roche Ltd. Data on file.