UC-TIB-DenV-GT (serotype 1-4)

For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-DenV-GT serotype 1-4 RUO UC-TIB-DenV-GT (serotype 1-4) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000858 10 340 096 001 10340096001 UC-TIB-DenV-GT UC-TIB-DenV-GT Reagents, kits 192 reactions Not Available false UC-TIB-DenV-GT is an automated qualitative in vitro test for the detection of Dengue Virus (DenV) RNA. The test utilizes amplification of target RNA by RT-PCR and nucleic acid hybridization for the detection and discrimination of the arthropod-born Dengue virus (DenV) serotypes 1-4. en The limit of detection (LoD) for UC-TIB-DenV-GT was determined by analysis of serial dilutions of the WHO International Standards for Dengue virus types 1 to 4 for NAT-based assays obtained from FDA, in DenV- negative human plasma (850 μL sample volume). The study demonstrates that the UC-TIB-DenV-GT detects DenV-1 and DenV-2 RNA at a concentration of 10 IU/mL and DenV-3 and DenV-4 RNA at a concentration of 20 IU/mL, determined by LOGIT analysis with a hit rate of 95%. en Viral RNA is analyzed by real-time PCR that amplifies a 130 bp long fragment of the 5 ́-UTR from DenV detected with a Coumarin-labeled probe in channel 01 for serotype 1, a FAM-labeled probe in channel 02 for serotype 2, a HEX-labeled probe in channel 03 for serotype 3 and a LC640 labeled probe in channel 04 for serotype 4. The (+) Ctrl comprises plasmids of synthetic 5 ́-UTR fragments with 275 bp from DenV-1, 277 bp from DenV- 2, 275 bp from DenV-3 and 279 bp from DenV-4 (Accession number: NC_001477 position 2 - 278 for DenV- 1, MK506263 position 2 - 280 for DenV-2, NC_001475 position 2 - 278 for DenV-3 and NC_002640 position 2 - 282 for DenV-4 respectively). en

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UC-TIB-DenV-GT (serotype 1-4)

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