For in vitro diagnostic use. Others VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail IVD VENTANA® Kappa and Lambda Dual ISH mRNA Probe Cocktail PID00000773 CE-IVD 08 507 023 001 8 507 023 001 08507023001 8507023001 08507023001 VENTANA K L DISH mRNA PRB CKT-USExport VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail 07613336160558 Reagents, kits 800-6054 30 tests Not Available true VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail está destinado a la detección cualitativa de ARNm Kappa y ARNm Lambda en tejido linfático y de médula ósea humanos fijado con formol y embebido en parafina (FFPE) teñido en un instrumento BenchMark IHC/ISH con hibridación in situ (ISH) cromogénica y cuya visualización se lleva a cabo mediante microscopía óptica. VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail se ha indicado como ayuda para la identificación de linfomas de linfocitos B y neoplasias de plasmocitos.La interpretación de los resultados del ensayo debe correr a cargo de un anatomopatólogo cualificado junto con un examen histológico, la información clínica pertinente y los controles adecuados.Este producto está destinado para uso diagnóstico in vitro (IVD). es VENTANA K/L Probe Cocktail is formulated for use with the VENTANA Magenta ISH DIG Detection Kit, the VENTANA Silver ISH BF Detection Kit, the ISH TSA Ancillary Kit, and accessory reagents on a BenchMark IHC/ISH instrument.The detection kits contain HRP-labeled anti-hapten primary antibody, hapten-labeled tyramide amplification reagent, and silver or magenta chromogen. During the ISH staining process, BF and DIG-labeled probes are hybridized to their respective target sequences in the tissue.DIG-labeled kappa probe is detected with VENTANA Magenta ISH DIG Detection Kit. This detection system uses HRP-labeled anti-DIG mouse monoclonal antibody and DIG- labeled tyramide amplification to visualize the target as a magenta signal through the covalent linkage of sulforhodamine B labeled tyramide to the tissue (see Figure 2).Figure 2. VENTANA Magenta ISH DIG Detection Kit BF-labeled lambda probe is detected with VENTANA Silver ISH BF Detection Kit. This detection system uses HRP-labeled anti-BF mouse monoclonal antibody and BF-labeled tyramide amplification to visualize the target as a black signal through the precipitation of silver (see Figure 3).Figure 3. VENTANA Silver ISH BF Detection Kit Refer to the method sheets of these detection reagents for additional information.The staining protocol consists of numerous steps in which reagents are incubated for pre- determined times at specific temperatures. At the end of each incubation step, the BenchMark instrument washes the sections to remove unbound material and applies a liquid coverslip to minimize evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope.For more detailed information on instrument operation, refer to the appropriate User Guide. en

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VENTANA^® Kappa and Lambda Dual ISH mRNA Probe Cocktail