For in vitro diagnostic use. Others cobas cfDNA Sample Preparation Kit IVD cobas® cfDNA Sample Preparation Kit RMD-4800-EGFRV2-001 07 247 737 190 7 247 737 190 07247737190 7247737190 07247737190 KIT COBAS CFDNA SAMP PREP 24T cobas cfDNA Sample Preparation Kit 00875197005424 Reagents, kits 1 kit 24 tests false The cobas® cfDNA Sample Preparation Kit is used for manual sample preparation to isolate circulating cell-free DNA (cfDNA) from plasma samples. en Sample preparation Plasma samples are processed and cfDNA isolated using the cobas® cfDNA Sample Preparation Kit, a generic manual sample preparation based on nucleic acid binding to glass fibers. Two milliliters (mL) of plasma are processed with a protease and a chaotropic binding buffer that protects the cfDNA from DNases. Subsequently, isopropanol is added to the binding mixture that is then centrifuged through a column with a glass fiber filter insert. During centrifugation, the cfDNA is bound to the surface of the glass fiber filter. Unbound substances, such as salts, proteins and other impurities, are removed by centrifugation. The adsorbed nucleic acids are washed and then eluted with an aqueous solution. en

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