For in vitro diagnostic use. Others EBER Epstein-Barr Virus Early RNA Probe INFORM IVD INFORM EBER (Epstein-Barr Virus Early RNA) Probe RTD000965 05 278 660 001 5 278 660 001 05278660001 5278660001 05278660001 INFORM EBER PROBE-US EXPORT INFORM EBER (Epstein-Barr Virus Early RNA) Probe 04015630971923 Reagents, kits 800-2842 50 tests Not Available true INFORM EBER (Epstein-Barr Virus Early RNA) Probe is intended for laboratory use in the qualitative in situ hybridization (ISH) detection of cells expressing Epstein-Barr encoded RNA (EBER) by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This product is intended for in vitro diagnostic (IVD) use. en INFORM EBER Probe is optimally formulated for use with VENTANA ISH iVIEW Blue Detection Kit and accessory reagents on a BenchMark IHC/ISH instrument.During the Blue ISH staining process, the fluorescein labeled probe is hybridized to specific target RNA sequences in tissues. This step is followed by the addition of a mouse anti-fluorescein antibody which binds to the hapten on the probe. This step is followed by the addition of a biotinylated goat anti-mouse secondary antibody which binds to the antifluorescein antibody. This step is followed by the addition of Streptavidin-AP (alkaline phosphatase) enzyme conjugate which binds to the biotin present on the secondary antibody. The fluorescein labeled probe is then visualized with 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) chromogen, which produces a blue precipitate that is readily detected by light microscopy.The staining protocol consists of numerous steps in which reagents are incubated for predetermined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instrument washes the sections to remove unbound material and applies a liquid coverslip which minimizes the evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope and may aid in the differential diagnosis of pathophysiological processes. Figure 2 illustrates the Blue ISH reaction. en

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