For in vitro diagnostic use. Others Kappa Probe INFORM IVD INFORM Kappa Probe RTD000971 05278678001 INFORM KAPPA PROBE-US EXPORT INFORM Kappa Probe 04015630972012 Reagents, kits 800-2843 50 tests Not Available true INFORM Kappa Probe is intended for laboratory use in the qualitative in situ hybridization-based detection of Kappa light chain mRNA by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This product is intended for in vitro diagnostic (IVD) use. en INFORM Kappa Probe is formulated for use with VENTANA ISH iVIEW Blue Detection Kit and accessory reagents on a BenchMark IHC/ISH instrument.During the Blue ISH staining process, the fluorescein labeled probe is hybridized to specific target RNA sequences in tissues. This step is followed by the addition of a mouse anti-fluorescein antibody which binds to the hapten on the probe. This step is followed by the addition of a biotinylated goat anti-mouse secondary antibody which binds to the anti-fluorescein antibody. This step is followed by the addition of Streptavidin-AP (alkaline phosphatase) enzyme conjugate which binds to the biotin present on the secondary antibody. The fluorescein labeled probe is then visualized with 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) chromogen, which produces a blue precipitate that is readily detected by light microscopy.The staining protocol consists of numerous steps in which reagents are incubated for pre-determined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instrument washes the sections to remove unbound material and applies a liquid coverslip which minimizes the evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with positive staining for the probe.Figure 1 illustrates the Blue ISH reaction.Figure 1. Blue ISH Reaction  en