For life science research only. Not for use in diagnostic procedures. Others LightMix Kit PAI-1 LSR LightMix® Kit PAI-1 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. TIBMOLBIOL-0057 Detection Kit for 4G/5G DNA variation in the PAI-1 gene 05 947 219 001 5 947 219 001 05947219001 5947219001 05947219001 LightMix PAI-4G/5G LightMix PAI-4G/5G Reagents, kits 40-0099-RUO 64 reactions Not Available false This kit detects the 4G/5G single-base deletion in the promotor region of the plasminogen-activator-inhibitor (PAI-1) gene from human genomic DNA extracts. A 345 bp fragment of the PAI-1 gene is amplified with specific primers. The PCR fragment is analyzed using an internally labeled SimpleProbe® (465-510 channel) binding to the region spanning the mutation site. For use on Roche LightCycler® systems. en Analytical SensitivityDetection of serial dilutions of several heterozygous human genomic DNAs has revealed that the limit of detection of the present kit is 250 copies (1.5 ng).Analytical SpecificityThe specificity to the target gene and the suitability of the PCR amplification employed in the present test was demonstrated by sequencing of the amplicon. en Using PCR methodology, a 120 bp fragment of the MTHFR gene is amplified with specific primers. The PCR fragment is analyzed using an internally labeled SimpleProbe® oligomer binding to the region spanning the mutation site.During the melting curve analysis the temperature is slowly increased. The probe leaves at a specific temperature (Tm) causing a fluorescence decrease. Any mismatch covered by the probe destabilizes the hybrid and lowers the Tm. In this product the probe matches the sequence of the wild type genotype and the presence of a mutation will yield a reduced Tm.Reading of the genotype results is based on the melting temperatures compared to the supplied standards. If permitted by the instrument software reading of the genotype results can be achieved by the automated genotyping module (instrument- dependent: software module 'Melt Curve Genotyping').Automated genotyping results must be reviewed by eye for deviating curves and intermediate melting point temperatures. In case that automated typing fails to report consistent genotype results, the genotype must be deduced from the melting temperatures following the criteria described in chapter 8. en

LightMix^® Kit PAI-1

Detection Kit for 4G/5G DNA variation in the PAI-1 gene

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