For in vitro diagnostic use. Cytoplasm Others Negative Control Rabbit Ig CONFIRM IVD CONFIRM Negative Control Rabbit Ig RTD000815 Rabbit 05266238001 CONFIRM Neg. Contr. Rabbit Ig CONFIRM Negative Control Rabbit Ig 04015630971947 Reagents, kits 760-1029 250 tests Not Available true CONFIRM Negative Control Rabbit Ig is intended for laboratory use as a control for non-specific binding of rabbit immunoglobulin in sections of formalin fixed, paraffin embedded tissue stained on a BenchMark IHC/ISH instrument.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This antibody is intended for in vitro diagnostic (IVD) use. en CONFIRM Negative Control Rabbit Ig may be used in place of the primary antibody as a negative control for immunohistochemical staining of formalin-fixed, paraffin-embedded (FFPE) tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and cover slipped. Results are interpreted using light microscopy.CONFIRM Negative Control Rabbit Ig is optimally diluted for use with VENTANA detection kits and BenchMark IHC/ISH instruments. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, liquid coverslip is applied in the instrument. Staining is completed after incubation with a substrate chromogen and optional counterstaining. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instrument User Guide. en