For in vitro diagnostic use. Others VENTANA Magenta ISH DIG Detection Kit IVD VENTANA® Magenta ISH DIG Detection Kit PID00000778 08 507 201 001 8 507 201 001 08507201001 8507201001 08507201001 VENTANA MAGENTA ISH DIG DETECTION KIT VENTANA Magenta ISH DIG Detection Kit 07613336160589 Reagents, kits 760-514 60 tests Not Available true The VENTANA Magenta ISH DIG Detection Kit is an indirect detection system for detecting DIG-labeled targets. The kit is intended to identify targets by chromogenic magenta in situ hybridization (ISH) in sections of formalin-fixed, paraffin-embedded tissue that are stained on a BenchMark IHC/ISH instrument.Staining results should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.The reagent is intended for in vitro diagnostic (IVD) use. en Following probe hybridization and stringency washes, target-bound digoxigenin (DIG) labeled probe is detected with HRP-labeled anti-DIG antibody. Signal amplification is accomplished through HRP catalyzed oxidation of tyramide-linked DIG by H2O2. The reactive oxidized form of tyramide covalently binds to tyrosine residues, increasing the local concentration of DIG hapten. This additional bound DIG is detected through a second application of the HRP labeled anti-DIG antibody. Finally, signal is visualized through another tyramide reaction involving tyramide-linked sulforhodamine B magenta dye.The staining protocol consists of numerous steps in which reagents are incubated for pre- determined times at specific temperatures. At the end of each incubation step, the BenchMark instrument washes the sections to remove unbound material and applies a liquid coverslip to minimize evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with positive staining for the probe.For more detailed information on instrument operation, refer to the appropriate User Guide. en

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VENTANA^® Magenta ISH DIG Detection Kit

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