For in vitro diagnostic use. Others VENTANA Silver ISH BF Detection Kit IVD VENTANA® Silver ISH BF Detection Kit PID00000779 08507031001 VENTANA SILVER ISH BF DETECTION KIT VENTANA Silver ISH BF Detection Kit 07613336160565 Reagents, kits 760-513 60 tests Not Available true The VENTANA Silver ISH BF Detection Kit is an indirect detection system for detecting BF-labeled targets. The kit is intended to identify targets by silver in situ hybridization (ISH) in sections of formalin-fixed, paraffin-embedded tissue that are stained on a BenchMark IHC/ISH instrument.Staining results should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controlsThe reagent is intended for in vitro diagnostic (IVD) use. en Following probe hybridization and stringency washes, target-bound benzofurazan (BF)- labeled probe is detected with HRP labeled anti-BF antibody. Signal amplification is accomplished through HRP catalyzed oxidation of tyramide linked BF by H2O2. The reactive, oxidized form of tyramide covalently binds to tyrosine residues, increasing the local concentration of BF hapten. This additional bound BF is detected through a second application of the HRP labeled anti-BF antibody. Signal is visualized through the reduction of soluble Ag+ to insoluble Ag0 by hydroquinone in a reaction catalyzed by HRP and H2O2.The staining protocol consists of numerous steps in which reagents are incubated for pre- determined times at specific temperatures. At the end of each incubation step, the BenchMark instrument washes the sections to remove unbound material and applies a liquid coverslip to minimize evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with positive staining for the probe.For more detailed information on instrument operation, refer to the appropriate User Guide. en