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Streamlined, automation-friendly DNA library preparation workflows
The KAPA DNA library preparation portfolio provides a complete, high-quality library prep solution for Illumina sequencing platforms. Our DNA library prep kits are built with enzymes and reaction buffers developed through proprietary Directed Evolution Technology, giving you everything you need for a seamless workflow–including available accessories like NGS adapters and cleanup beads. From DNA input to library amplification and QC, we’ve got you covered.
Customize your workflows without compromise, starting with a wide variety of DNA input amounts and sample types
Preserve the integrity of your sequencing libraries with reduced PCR amplification bias
Choose your DNA fragmentation method, using enzymatic fragmentation or mechanically fragmented DNA
Use a variety of workflow setups, including automation
- Ensure your success with expert, end-to-end support for your NGS sample prep workflow
DNA library preparation for next-generation sequencing (NGS) involves the fragmentation of target DNA molecules into varying sizes. The KAPA library prep portfolio offers kits for both enzymatic fragmentation and mechanically fragmented DNA. The fragmented DNA is then subjected to end repair/A-tailing and ligation of platform-specific adapters to the library.
The libraries are subsequently amplified (in with-PCR workflows) and cleaned up using beads (this may also include size-selection steps). Depending on the nature of the DNA sample–for example, a fresh sample versus a formalin-fixed paraffin-embedded (FFPE) tissue–the workflow may require optimization.
Featured products
RUO
For Research Use Only. Not for use in diagnostic procedures.
KAPA EvoPlus Kits
KAPA EvoPlus Kits offer reliable, high-performance DNA library preparation with enhanced enzymatic fragmentation and a streamlined, fully automatable workflow.
RUO
For Research Use Only. Not for use in diagnostic procedures.
KAPA EvoPrep Kits
KAPA EvoPrep Kits enable pre-fragmented DNA library prep for WGS, WES, targeted sequencing, and methyl-seq with a streamlined, fully automatable workflow.
RUO
For Research Use Only. Not for use in diagnostic procedures.
KAPA Adapters
Full-length adapters compatible with ligation-based library construction research workflows for sequencing applications on the Illumina® platform.
Benefits of DNA library preparation solutions from Roche
Broad compatibility
Customize your workflows without compromise
KAPA DNA library prep kits support a broad range of DNA input sample types, such as high-quality genomic DNA or challenging materials like FFPE or cfDNA.
Start with a wide variety of DNA inputs (DNA, cfDNA, FFPE)
Choose your preferred DNA fragmentation method: mechanical shearing or enzymatic fragmentation
Begin with as little as 0.1 ng input DNA
Low bias
Preserve the integrity of your DNA libraries during amplification
Advanced formulations in KAPA kits reduce the introduction of bias during DNA library preparation, making NGS data more reliable.
Reduce amplification bias with KAPA EvoAmp ReadyMix—while saving valuable time and reducing pipetting steps
Achieve greater sequencing coverage1 compared to other suppliers due to lower bias
Convenient formats
Use a variety of workflow setups, including automation
The wide variety of kit configurations in the KAPA portfolio, including automation-friendly plated formats, supports a broad range of throughput needs and NGS applications.
Choose either PCR-free or with-PCR reagent modules
Select from various reaction sizes and configurations, including tubes or plated reagents
Maximize convenience and save time with ultra-pure, high-quality library construction reagents in convenient master-mix and ReadyMix formats
Integrated solution
Ensure your success with expert, end-to-end support
Work seamlessly with downstream applications, reducing sample transfers and increasing confidence in NGS results. From fragmentation and ligation to target enrichment, the Roche Support Network and KAPA DNA library preparation workflows have you covered.
Order everything you need in one place, including modules for fragmentation (with certain kits), end repair, A-tailing, ligation, and amplification. Also available are KAPA adapters and cleanup beads.
Add target enrichment to your workflow with KAPA HyperCap or KAPA HyperPETE workflows for a complete integrated sample prep solution
KAPA DNA library prep kits comparison overview
KAPA EvoPlus Boost Kit
|
KAPA EvoPlus V2 Kit, PCR-free
|
KAPA EvoPrep Boost Kit
|
KAPA EvoPrep Kit, PCR-free
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| Recommended sample types | |||
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Genomic DNA, FFPE DNA
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Genomic DNA
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Genomic DNA, cfDNA, FFPE DNA
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Genomic DNA
|
| Input range | |||
|
100 pg - 500 ng
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50 ng* - 500 ng
|
100 pg - 500 ng
|
50 ng* - 500 ng
|
| Fragmentation method (if required) | |||
|
Enzymatic fragmentation (integrated)
|
Enzymatic fragmentation (integrated)
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Mechanical shearing
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Mechanical shearing
|
| Library Amplification reagent | |||
|
KAPA EvoAmp ReadyMix, powered by HiFi
|
None
|
KAPA EvoAmp ReadyMix, powered by HiFi
|
None
|
| Adapter compatibility | |||
|
KAPA UDI Adapters, KAPA HyperPlex Adapters
|
KAPA UDI Adapters
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KAPA UDI Adapters, KAPA HyperPlex Adapters
|
KAPA UDI Adapters
|
| Key applications | |||
|
WGS, WES, targeted sequencing
|
WGS
|
WGS, WES, and targeted sequencing Metyl-seq
|
WGS
|
*Dependent upon input sample type, amount, and quality.
Automation-friendly DNA library preparation
Thinking of automating library preparation? Roche provides NGS automation support with a menu of automated methods for KAPA library prep reagents on non-Roche liquid handling vendors, and on Roche's AVENIO Edge System.
KAPA products are for Research Use Only. Not for use in diagnostic procedures.
The AVENIO Edge System is a Class 1 US IVD and a Class A CE IVD (under IVDR) and is intended for downstream diagnostic applications. For the EU, Roche is collaborating with Platomics to enable laboratories to help automate document generation on Platomics’ multi-stakeholder platform for workflows incorporating the Avenio Edge System. Reagents and kits mentioned are for Research Use Only. They are not intended for diagnostic procedures. The AVENIO Edge System can be used for Research Use Only (RUO) workflows.
References
F. Hoffmann-La Roche Ltd. Data on file.