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For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-GAS Streptococcus pyogenes RUO UC-TIB-GAS (Streptococcus pyogenes) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly.
Furthermore we can not guarantee that all available information for this product is up to date. PID00000703 10 097 744 001 10097744001 UC-TIB-GAS UC-TIB-GAS 04260159335040 Reagents, kits 192 reactions Not Available false 10 175 397 001 10175397001 UC-TIB-GAS 10x UC-TIB-GAS 10x 04260159335613 Reagents, kits 10 x 192 reactions Not Available undefined UC-TIB-GAS is an automated qualitative in vitro test for the detection of Streptococcus pyogenes (GAS) DNA from putative phosphoenolpyruvate sugar PTS enzyme I gene.
Based on: Comparison of LightCycler PCR, Rapid Antigen Immunoassay, and Culture for Detection of Group A Streptococci from Throat Swabs. Uhl et al., 2003 en Bacterial DNA is analyzed by a real-time PCR that amplify a 95 bp long fragment of PTS enzyme I gene, in channel 02 using a FAM-labelled probe.
The (+) Ctrl comprise a plasmid with a 324 bp long synthetic fragment of PTS enzyme I gene (Accession number: CP000261 position 1148809 - 1149134). en The limit of detection (LoD) for UC-TIB-GAS was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200 μL sample volume). The study demonstrates that the UC-TIB-GAS detects at least 85 genome equivalent copies or less per mL, determined by LOGIT analysis with a hit rate of 95%. en