UC-TIB-Gastro-BAC (Salm./Yers./Camp./Shig.)

For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Gastro-BAC RUO UC-TIB-Gastro-BAC (Salm./Yers./Camp./Shig.) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000709 10 175 265 001 10175265001 UC-TIB-Gastro-BAC UC-TIB-Gastro-BAC 04260159335217 Reagents, kits 192 reactions Not Available false 10 175 290 001 10175290001 UC-TIB-Gastro-BAC 10x UC-TIB-Gastro-BAC 10x 04260159335552 Reagents, kits 10 x 192 reactions Not Available false UC-TIB-Gastro-BAC is an automated qualitative in vitro test for the detection of gastro-bacterial DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of Salmonella spp., Yersinia enterocolitica/pseudotuberculosis, Campylobacter spp. and Shigella spp.. en Bacterial DNA is analyzed by real-time PCR, that amplifies a 85 bp long fragment of ttrR gene from Salmonella spp. analyzed with a Coumarin-labeled probe in channel 1, a 98 bp long fragment of foxA gene from Yersinia enterocolitica and a 95 bp long fragment of invA gene from Yersinia pseudotuberculosis analyzed with FAM-labeled probes in channel 2, a 98 bp long fragment of 16S rRNA gene from Campylobacter spp. analyzed with a HEX-labeled probe in channel 3 and a 85 bp long fragment of ipaH gene from Shigella spp. analyzed with a JA270 labeled probe in channel 4. The (+) Ctrl comprises plasmids with a 165 bp long synthetic fragment of ttrR gene from Salmonella enterica, a 363 bp long synthetic fragment of invA gene from Yersinia pseudotuberculosis, a 1053 bp long synthetic fragment of 16S rRNA gene from Campylobacter jejuni and a 684 bp long synthetic fragment of ipaH gene from Shigella fexneri (Accession number: AF 282268 position 4267 - 4432 for ttrR gene from Salmonella enterica, CP045258 position 1668636 - 1668998 for invA gene from Yersinia pseudotuberculosis, CP000025 position 37801 - 38853 for 16S rRNA gene from Campylobacter jejuni and AY206436 position 859 - 1543 for ipaH gene from Shigella fexneri respectively). en The limit of detection (LoD) for UC-TIB-Gastro-BAC was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200μL sample volume). The study demonstrates that the UC-TIB-Gastro-BAC detects at least 85 genome equivalent copies or less per mL for Salmonella spp., 100 copies or less per mL for Yersinia enterocolitica/pseudotuberculosis, Campylobacter spp. and Shigella spp., determined by LOGIT analysis with a hit rate of 95%. In rare cases gastroenteritis can be caused by Campylobacter fetus or Campylobacter ureolyticus, these Campylobacter species will not be detected! en

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