UC-TIB-Gastro-PARA (Entam./Giard./Crypto./Strong.)

For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Gastro-PARA RUO UC-TIB-Gastro-PARA (Entam./Giard./Crypto./Strong.) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000706 10 175 320 001 10175320001 UC-TIB-Gastro-PARA UC-TIB-Gastro-PARA 04260159335347 Reagents, kits 192 reactions Not Available false 10 175 338 001 10175338001 UC-TIB-Gastro-PARA 10x UC-TIB-Gastro-PARA 10x 04260159335583 Reagents, kits 10 x 192 reactions Not Available false UC-TIB-Gastro-PARA is an automated qualitative in vitro test for the detection of gastrointestinal parasitic DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of Entamoeba histolytica (E.hist), Giardia intestinalis (G.intes), Cryptosporidium spp. (Crypto) and Strongyloides stercoralis (S.sterco). en Parasitic DNA is analyzed by real-time PCR, that amplifies a 104 bp long fragment of 18S rRNA gene from E.hist analyzed with a Coumarin-labeled probe in channel 1, a 110 bp long fragment of gdh gene from G.intes analyzed with a FAM-labeled probe in channel 2, a 118 bp long fragment of COWP gene from Crypto analyzed with a HEX-labeled probe in channel 3 and a 99 bp long fragment of 18S rRNA gene from S.sterco analyzed with a JA270 labeled probe in channel 4. The (+) Ctrl comprises plasmids with a 411 bp long synthetic fragment of 18S rRNA gene from E.hist, a 175 bp long synthetic fragment of gdh gene frm G.intes, a 952 bp long synthetic fragment of COWP gene from Crypto and a 354 bp long synthetic fragment of 18S rRNA gene from S.sterco (Accession number: X64142 position 87 - 498 for 18S rRNA gene from E.hist, MK645799 position 275 - 449 for gdh gene from G.intes, AB514066 position 1 - 952 for COWP gene from Crypto and M84229 position 1373 - 1729 for 18S rRNA gene from S.sterco respectively). en The limit of detection (LoD) for UC-TIB-Gastro-PARA was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200 μL sample volume). The study demonstrates that the UC-TIB- Gastro-PARA detects at least 60 genome equivalent copies or less per mL for E.hist, 100 copies or less per mL for G.intes, 50 copies or less per mL for Crypto and 80 copies or less per mL for S.sterco, determined by LOGIT analysis with a hit rate of 95%. en

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UC-TIB-Gastro-PARA (Entam./Giard./Crypto./Strong.)

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