UC-TIB-Gastro-Virus (AdV-F/RoV-A/NoroV/AstroV)

For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Gastro-Virus RUO UC-TIB-Gastro-Virus (AdV-F/RoV-A/NoroV/AstroV) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000705 10 175 346 001 10175346001 UC-TIB-Gastro-Virus UC-TIB-Gastro-Virus 04260159335354 Reagents, kits 192 reactions Not Available false 10 175 354 001 10175354001 UC-TIB-Gastro-Virus 10x UC-TIB-Gastro-Virus 10x 04260159335590 Reagents, kits 10 x 192 reactions Not Available false UC-TIB-Gastro-Virus is an automated qualitative in vitro test for the detection of gastrointestinal viral nucleic acid. The test utilizes amplification of target DNA and RNA by RT-PCR and nucleic acid hybridization for the detection of human Adenovirus F (AdV-F) type 40 and 41, human Rotavirus A (RoV-A), human Norovirus GGI & GGII (NoV) and human Astrovirus (AstV). en For AdV-F a dual-target PCR, targeting the F-group specific fiber protein gene, is used. One amplicon is a 91 bp (type 40) or 88 bp (type 41) long fragment, the second PCR is a 68 bp amplicon both from the long fiber protein gene, detected each with Coumarin-labeled probes in Channel 01. Viral RNA is analyzed by real-time PCR, that amplifies a 87 bp long fragment of NSP5 gene (segment 11) from RoV-A detected with a FAM-labeled probe in channel 2, a 70 bp (GGI) or 95 bp (GGII) long fragment of the junction sequence between the orf1 and orf2 genes from NoV detected with HEX-labeled probes in channel 3 and a 103 bp long fragment of the viral capsid gene from AstV analyzed with a JA270 labeled probe in channel 4. The (+) Ctrl comprises plasmids with a 363 bp long synthetic fragment of fiber protein gene from AdV-F, a 662 bp long synthetic fragment of NSP5 gene from RoV-A, a 475 bp long synthetic fragment of junction sequence between the orf1 and orf2 genes from NoV GGII and a 660 bp long synthetic fragment of capsid gene from AstV (Accession number: AB610545 position 316 - 678 for fiber protein gene from AdV-F, JX682996 position 3 - 663 for NSP5 gene from RoV-A, KY817495 position 439 - 914 from NoV GGII and AY720892 position 4323 - 4983 for capsid gene from AstV respectively). en The limit of detection (LoD) for UC-TIB-Gastro-Virus was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200 μL sample volume). The study demonstrates that the UC-TIB- Gastro-Virus detects at least 130 genome equivalent copies or less per mL for AdV-F, 270 copies or less per mL for RoV-A, 1,000 copies or less per mL for NoV GGI and 140 copies or less per mL for NoV GGII, and 170 copies or less per mL for AstV, determined by LOGIT analysis with a hit rate of 95%. en

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UC-TIB-Gastro-Virus (AdV-F/RoV-A/NoroV/AstroV)

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