UC-TIB-HSV/VZV (RUO)

For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-HSV VZV RUO RUO UC-TIB-HSV/VZV (RUO) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. RMD-UC-004 09 838 929 001 9 838 929 001 09838929001 9838929001 09838929001 UC-TIB-HSV/VZV-RUO UC-TIB-HSV/VZV-RUO Reagents, kits 1 kit 192 tests 10 336 528 001 10336528001 UC-TIB-HSV/VZV-RUO UC-TIB-HSV/VZV-RUO 04260159336108 Reagents, kits 1 kit 192 tests false The limit of detection (LoD) of UC-TIB-HSV/VZV (RUO) was determined by analysing serial dilutions of the HSV-1 (MacIntyre strain), HSV-2 (MS strain) and VZV (82 strain) strains obtained from Zeptometrix, in HSV/VZV-negative human EDTA plasma (200 μL sample volume). These strains were quantified in c/mL with a calibration curve using quantified genomic DNA from ATCC as standard solutions. The study demonstrates that UC-TIB-HSV/VZV (RUO) detects HSV-1, HSV-2 and VZV DNA at respective concentrations of 57 c/mL, 68 c/mL and 75 c/mL, determined by PROBIT with a hit rate of 95%. en UC-TIB-HSV/VZV (RUO) is an automated qualitative in vitro test for the detection of human Herpes Simplex Virus-1 (HSV-1), Herpes Simplex Virus-2 (HSV-2) and Varicella Zoster Virus (VZV) DNA. This test utilizes the open channel functionality (cobas® omni utility channel) of the cobas® x800 Systems. en Viral DNA is analyzed by a real-time PCR that amplify a 108 bp long fragment of UL5 gene from HSV-1 analyzed with a FAM-labeled probe in channel 02, a 146 bp long fragment of envelope glycoprotein B from VZV analyzed with a HEX-labeled probe in channel 03, and a 106 bp long fragment of UL30 gene from HSV-2 analyzed with a Cal Fluor Red 635 labeled probe in channel 04.The (+) Ctrl comprises plasmids with a 467 bp long synthetic fragment of UL5 gene from HSV-1, a 240 bp long synthetic fragment of envelope glycoprotein B from VZV and a 516 bp long synthetic fragment of UL30 gene from HSV-2 (Accession number: MK878601 position 1432 - 1898 for UL5 gene from HSV-1, X04370 position 57738 - 57981 for envelope glycoprotein B from VZV and M16321 position 1580 - 2095 for UL30 gene from HSV-2 respectively). en

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