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For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-LGV RUO UC-TIB-LGV (Chlamydia trachomatis, LGV) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly.
Furthermore we can not guarantee that all available information for this product is up to date. PID00000710 10 097 787 001 10097787001 UC-TIB-LGV UC-TIB-LGV 04260159334982 Reagents, kits 192 reactions Not Available false UC-TIB-LGV is an automated qualitative in vitro test for the detection of Chlamydia trachomatis (CT) DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of CT and differentiate the strains (L1, L2a, L2b and L3) causing Lymphogranuloma venereum (LGV) from non LGV strains. en Bacterial DNA is analyzed by real-time PCR, that amplifes a 79 bp long fragment of polymorphic membrane protein H (pmpH) gene, in channel 02 using a FAM-labelled probe for CT non LGV and channel 03 using a HEX-labelled probe specific for CT invasive serovars L1, L2a, L2b and L3 (LGV) strains.
The (+) Ctrl comprises plasmids with a 331 bp long synthetic fragment of pmpH gene from a LGV strain, and a 401 bp long synthetic fragment of pmpH gene from a non-LGV strain (Accession number: MG542649 position 3 - 334 or 3 - 405 respectively). en The limit of detection (LoD) for UC-TIB-LGV was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (400 μL sample volume). The study demonstrates that the UC-TIB-LGV detects at least 50 genome equivalent copies or less per mL, determined by LOGIT analysis with a hit rate of 95%. en