UC-TIB-NG_Resistance

For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-NG Resistance RUO UC-TIB-NG_Resistance 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001016 10 490 039 001 10490039001 UC-TIB-NG_Resistance UC-TIB-NG_Resistance Reagents, kits 192 reactions Not Available false UC-TIB-NG_Resistance test is an automated qualitative in vitro test for the amplification and detection of Neisseria gonorrhoeae (NG) pilin inversion gene (piv) and the gyrase subunit A (gyrA) gene mutation (S91F) associated with Ciprofloxaxin resistance/susceptibility. en NG DNA is detected by real-time PCR, that amplifies a 154 bp long fragment of the piv gene in channel 04 using a LC640 labelled probe. Ciprofloxacin resistance/susceptibility is detected by real-time PCR, that amplifies a 104 bp fragment of the gyrA gene. The S91 variant (Ciprofloxacin susceptible) is detected in channel 02 using a FAM labelled probe and the 91F variant (Ciprofloxacin resistant) is detected in channel 03 using a HEX labelled probe. The positive control comprises plasmids with a 229 bp long synthetic fragment of piv gene, a 432 bp long synthetic fragment of gyrA gene S91 variant and a 432 bp long synthetic fragment of gyrA gene 91F variant (Accession number: AP023073 position 1720104 - 1720332 for piv gene and U08817 position 73 - 505 for gyrA gene respectively). en The limit of detection (LoD) for UC-TIB-NG_Resistance was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media. The study demonstrates that the UC-TIB-NG_Resistance detects NG at a concentration of 120 copies/mL, determined by LOGIT analysis with a hit rate of 95%. en

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