UC-TIB-TBEV/Borrelia

For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-TBEV Borrelia RUO UC-TIB-TBEV/Borrelia PID00000819 10 280 603 001 10280603001 UC-TIB-TBEV/Borrelia UC-TIB-TBEV/Borrelia Reagents, kits 192 reactions Not Available false UC-TIB-TBEV/Borrelia is an automated qualitative in vitro test for the detection of Tick-Borne Encephalitis Virus (TBEV) RNA, as well as Borrelia spp. DNA. This product detects TBEV including other members of the TBEV serocomplex like the Oms Hemorrhagic Fever Virus (OHFV), Louping Ill Virus (LIV), Langat Virus (LaV) and Powasan Virus (PowV). Kyasanur Forest Disease Virus (KFDV) and Alkhurma Virus (AlkV) are not amplified, based on in-silico analysis. The Borrelia spp. detected, include Borrelia burgdorferi sensu stricto, B. miamotoi, B. afzelii, B. spielmanii, B. garinii, B. lusitaniae and others. en For TBEV RNA a 67 bp amplicon from the 3 ́-UTR is amplified and detected with Coumarin-labeled probes in channel 01. Borrelia spp. DNA is analyzed amplifying a 74 bp long fragment of the 23S rRNA gene with a FAM-labeled probe in channel 02 and as reference for Ixodidae spp. a 67 bp long fragment of the 5.8 rRNA gene is amplified and detected with a HEX-labeled probe in channel 03. The (+) Ctrl comprises plasmids with a 68 bp long synthetic fragment of 3 ́-UTR of TBEV and a 403 bp long synthetic fragment of 23S rRNA gene of Borrelia spp. (Accession number: AH002418 position 756 - 823 for 3 ́-UTR of TBEV and NR_076348 position 585 - 990 for 23S rRNA gene of Borrelia spp. respectively). en The limit of detection (LoD) for UC-TIB-TBEV/Borrelia was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (400 μL Sample volume). The study demonstrates that the UC-TIB-TBEV/Borrelia detects at least 100 genome equivalent copies or less per mL for TBEV and 42 copies or less per mL for Borrelia spp., determined by LOGIT analysis with a hit rate of 95%. en

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UC-TIB-TBEV/Borrelia

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