UC-TIB-VRE (vanA/B)

For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-VRE vanAB RUO UC-TIB-VRE (vanA/B) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000861 10 356 812 001 10356812001 UC-TIB-VRE UC-TIB-VRE Reagents, kits 192 reactions Not Available false The limit of detection (LoD) for UC-TIB-VRE was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200 μL sample volume). The study demonstrates that the UC-TIB-VRE detects at least 60 genome equivalent copies or less per mL for vanA or vanB, determined by LOGIT analysis with a hit rate of 95%. en UC-TIB-VRE is an automated qualitative in vitro test for the detection of gastro-bacterial DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of vanA and vanB gene of Enterococcus spp.. en Bacterial DNA is analyzed by real-time PCR that amplifies a 104 bp long fragment of vanA gene with a FAM- labelled probe in channel 02 and a 116 bp long fragment of vanB gene with a HEX-labelled probe in channel 03.The (+) Ctrl comprises plasmids with a 892 bp long synthetic fragment of vanA gene and a 734 bp long synthetic fragment of vanB gene (Accession number: AB563188 position 61660 - 62553 for vanA gene, and U94526 position 6 - 739 for vanB gene respectively). en

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