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Optimize your sequencing resources with custom NGS targeted enrichment panels
NGS custom panels empower you to interrogate a select subset of genomic regions at the breadth and depth required by your research goals—without wasting sequencing reads on other regions. However, without the right tools, the design and creation of custom NGS panels can be daunting; even worse, poorly designed panels can waste valuable time, samples, and other resources.
The online HyperDesign Tool from Roche takes the guesswork out of custom NGS panel design. HyperDesign simplifies panel design and delivers high-performing probe panels without the need for extensive empirical optimization. HyperDesign Tool is:
- created and supported by expert designers
- free
- user-friendly
HyperDesign Tool can design custom NGS panels for both of our target enrichment workflows, with custom probe panels for our KAPA HyperCap Workflow and custom primers/probes for our KAPA HyperPETE Workflow. For maximum flexibility, panels for both human and non-human genomes are available in a wide range of target sizes and product formats.
Featured products
RUO
For Research Use Only. Not for use in diagnostic procedures.
HyperDesign
HyperDesign is a new custom design tool that combines our KAPA Target Enrichment technology with Roche's renowned expertise in probe and primer design
RUO
For Research Use Only. Not for use in diagnostic procedures.
KAPA HyperCap Custom Probes
KAPA HyperCap Custom Probes are target enrichment probes with high fidelity and improved target capture NGS, utilizing the Roche probe design algorithm.
RUO
For Research Use Only. Not for use in diagnostic procedures.
KAPA HyperPETE Custom Probes
The KAPA HyperPETE Custom Probes panels, KAPA HyperPETE Choice Panels and KAPA HyperPETE Explore Panels, offer target enrichment of custom DNA and RNA regions.
Benefits of NGS target enrichment solutions from Roche
User-friendly design
Create successful NGS panel designs with expert-designed algorithms
Roche's online HyperDesign Tool was created to address the challenges of other design tools; while many others are clunky and challenging to set up, use poorly optimized design algorithms, or lack the support of a live person, the HyperDesign Tool is:
A user-friendly online probe design tool that takes advantage of Roche's two decades of in silico probe design experience
Supported by expert designers who are available if you need additional help
Driven by proprietary design algorithms that enable coverage of GC- or AT-rich regions, as well as other challenging regions that may contain important variants—without significantly increasing sequencing data requirements
The HyperDesign Tool may also be used to customize the content of pre-designed KAPA HyperCap or KAPA HyperPETE Design Share Panels if additional content is needed to satisfy your research goals.*
Targeted performance
Optimized design meets meticulous manufacturing to deliver NGS success
Both panel design and reagent quality—including the target enrichment probes—are essential in achieving optimal performance metrics from custom NGS panels.
- NGS custom panels designed with the HyperDesign Tool are pre-optimized for Roche target enrichment chemistries, and designed to deliver uniform coverage and low duplication rates—from the first iteration
- Probe manufacturing using KAPA HiFi DNA Polymerase yields high-fidelity probes that support very high levels of enrichment
- Once the Custom NGS Panels are created by Roche, they are subjected to NGS-based quality control testing to confirm the presence and concentration of probes/primers before they are packaged and sent to your lab
Flexibility
Flexibility matters
Custom targeted sequencing can be complicated if your NGS vendor doesn’t support your organism of interest, or offers limited product formulations that don’t meet your project requirements. Roche offers a variety of custom target enrichment options:
KAPA HyperCap Choice Panels for custom human designs, covering from 0.5 Mb - 200 Mb (pack sizes from 12 - 100,000 reactions)*
KAPA HyperCap Explore Panels for non-human genomes and unconventional human designs, enabling enrichment of up to 200 Mb (pack sizes from 12 - 100,000 reactions)**
KAPA HyperPETE Choice Panels for custom human designs (DNA or RNA inputs); target size of 75 kb - 250 kb for DNA and up to 50 kb panels for RNA (pack sizes from 96 - 10,000 reactions). KAPA HyperPETE Explore Panels offer similar product configurations for non-human genomes or unconventional human designs.**
Custom NGS Panels comparison overview**
View full tableCustom NGS Panels comparison overview**
|
KAPA HyperCap Choice Panels: |
KAPA HyperCap Explore Panels |
KAPA HyperPETE Choice Panels |
KAPA HyperPETE Explore Panels |
Genomes supported |
Human |
Non-human, unconventional human designs |
Human |
Non-human, unconventional human designs |
Target sizes supported |
0.5 - 200 Mb in fixed maximum size increments |
0.5 - 200 Mb in fixed maximum size increments |
75 - 250 kb (DNA) in fixed maximum size increments 50 kb (RNA) |
75 - 250 kb (DNA) in fixed maximum size increments 50 kb (RNA) |
Available pack sizes |
12 - 100,000 rxns in fixed rxn increments |
12 - 100,000 rxns in fixed reaction increments |
96, 385, 1536, or 10,000 rxns |
96, 385, 1536, or 10,000 rxns |
KAPA products are for Research Use Only, not for use in diagnostics procedures.
*Not all DesignShare Panels are currently available or supported for all workflows in all regions/countries. Restrictions apply to the customization of Design Share content.
**Not all target sizes and pack sizes are available in all regions/countries.