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Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "CONFIRM anti-MART-1/melan A (A103) contains sufficient reagent for 50 slides.

One 5 mL dispenser of CONFIRM anti-MART-1/melan A (A103) contains approximately 17 μg of a mouse monoclonal antibody.
The antibody is diluted in 0.05 M Tris-HCI with 2% carrier protein, and 0.10% ProClin 300, a preservative.
Specific antibody concentration is approximately 3.4 µg/mL. There is no known irrelevant antibody reactivity observed in this product.
CONFIRM anti-MART-1/melan A (A103) antibody is a mouse monoclonal antibody produced as a cell culture supernatant.
Refer to the appropriate VENTANA detection kit method sheet for detailed descriptions of: Principle of the Procedure, Material and Methods, Specimen Collection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Results, and Limitations.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "CONFIRM anti-MART-1/melan A (A103) Mouse Monoclonal Primary Antibody is intended for laboratory use in the qualitative immunohistochemical detection of melan A by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.
This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This antibody is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "Melan A, also known as melanoma antigen recognized by T-cells-1 (MART-1), is an ~18 kDa single-domain transmembrane protein that encodes a polypeptide of 118 amino acids. Melan A is located mainly in endoplasmic reticulum, melanosomes, and the transGolgi network.1,2 In normal human cells and tissues, melan A mRNA is expressed exclusively in melanocytes of the skin and retina.3,4 Neoplasms derived from these cell types also express melan A, such as primary and metastatic melanomas. Although melan A function is not fully elucidated, it is known to play an essential role in regulating pigmentation. Melan A forms a complex with premelanosome protein (PMEL), a protein essential in the formation of early melanosomes.5 Melan A impacts PMEL expression, stability, trafficking, and processing, which is required for melanosome structure and maturation.5 Detection of melan A protein by immunohistochemistry (IHC) with the CONFIRM antiMART-1/melan A (A103) antibody may be used as a melanocyte marker to aid in the differential diagnosis of melanocytic versus non-melanocytic tumors. It may be used as part of a panel of IHC studies. The staining pattern is cytoplasmic.

1. De Maziere AM, Muehlethaler K, van Donselaar E, et al. The melanocytic protein Melan-A/MART-1 has a subcellular localization distinct from typical melanosomal proteins. Traffic. 2002;3(9):678-693.
2. Rimoldi D, Muehlethaler K, Salvi S, et al. Subcellular localization of the melanoma-associated protein Melan-AMART-1 influences the processing of its HLA-A2- restricted epitope. J Biol Chem. 2001;276(46):43189-43196.
3. Coulie PG, Brichard V, Van Pel A, et al. A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. J Exp Med. 1994;180(1):35-42.
4. Kawakami Y, Eliyahu S, Delgado CH, et al. Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection. Proc Natl Acad Sci U S A. 1994;91(14):6458-6462.
5. Hoashi T, Watabe H, Muller J, Yamaguchi Y, Vieira WD, Hearing VJ. MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes. J Biol Chem. 2005;280(14):14006-14016.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Product Purpose", "Value": "CONFIRM anti-MART-1/melan A (A103) Mouse Monoclonal Primary Antibody is intended for laboratory use in the qualitative immunohistochemical detection of melan A by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.
This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This antibody is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }

CONFIRM anti-MART-1/melan A (A103) Mouse Monoclonal Primary Antibody

IVD For in vitro diagnostic use.
CONFIRM anti-MART-1/melan A (A103) Mouse Monoclonal Primary Antibody

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