Digital LightCycler® System

Digital PCR with the Digital LightCycler® system’s high-resolution 100,000 plate offers laboratories:

• The ability to more likely discriminate small differences between samples
• Superior cluster tightness and clarity of results
• Absolute quantification of results

The Digital LightCycler® system is designed to provide flexibility and efficiency, offering features that streamline workflows and adapt to a variety of laboratory needs:

• Choose batch size increments of between 8 and 96 samples per run
• Combine lanes for the results of a single sample to increase resolution
• Simplify workflow with sample tracking and automated data analysis
• Enable high multiplexing with a 6 optical channel analyzer (Demonstrated with both DNA and RNA reagents)
• Address multiple challenges at once with three plates for different application needs (Universal, High Sensitivity, and High Resolution)
• Analyzer run times vary for different batch sizes to save time on smaller batches
• Support assay development with a flexible platform suited for evolving research needs

The Digital LightCycler® System has an enhanced ability to detect minimal DNA or RNA molecules:

• High-sensitivity 20,000 partition plate w/ 45μl reaction volume
• 5x concentrated master mixes (RNA, DNA) enable high sample utilization, which means more confidence in results
• Little rain and clear cluster separation means setting thresholds is easy

Bringing it all together, the Digital LightCycler® System integrates advanced reagents, smart digital connectivity, and robust hardware design to deliver reliable, reproducible results.

• Chemistry of generic reagents:

  • 5x concentrated master mixes for greater potential sample volume input

  • DNA and RNA optimized for multiplexing

  • Filling dye control that validates partitioning

• 6 optical channels
• Nanowell plate types: solid phase partitioning instead of droplets increases reliability and replicability
• Closed system minimizes the chance of amplicon contamination
• Laboratory Information Digital LightCycler® system connectivity means a simple digital workflow and sample tracking
• Algorithm for sophisticated rejection of non-PCR artifacts
• Absolute quantification without the need for standard curves
• Unambiguous thresholding with clear cluster separation of positives and negatives, and very little rain

The unique hexagonal-shaped nanowell partitions and the filling dye that validates partitioning give the Digital LightCycler® system the ability to handle a wide variety of extracted samples, such as FFPET, cfDNA, Whole Blood, and more.

Through these partitions, target sequences are efficiently concentrated within isolated microreactors. This concentration effect reduces template competition, enabling the detection of rare mutations and allowing for a higher tolerance to inhibitors present in a sample.

Get the most out of your samples with the Digital LightCycler® System's 5x concentrated master mixes.

• Improved sample percentage in each reaction means confidence in results
• 1 Step RNA (RT) with a hold time of just 15 minutes
• DNA and RNA optimized for multiplexing
• Filling dye control that validates partitioning
• Single tube
• 50-75% less primer/probe usage

Get the most out of your samples with the Digital LightCycler® system's 5x concentrated master mixes.^1-4

• Improved sample percentage in each reaction means confidence in results
• 1 Step RNA (RT) with a hold time of just 15 minutes
• Filling dye control that validates partitioning
• Single tube
• 50-75% less primer/probe usage

The Digital LightCycler® system guides users through a streamlined PCR workflow while enabling sample tracking via its integrated web application.