For Research Use Only. Not for use in diagnostic procedures. Set KAPA HyperExome V2 Probes and Kits RUO KAPA HyperExome V2 Probes and Kits PID00001174 Target enrichment probes for exome capture 09 075 810 001 9 075 810 001 09075810001 9075810001 09075810001 KAPA HyperCapture Reagent Kit, 24 rxn KAPA HyperCapture Reagent kit 07613336198186 Reagents, kits 24 reactions Not Available 09 075 763 001 9 075 763 001 09075763001 9075763001 09075763001 KAPA Hybrid Enhancer Reagent, 1 ml KAPA Hybrid Enhancer Reagent 07613336198216 Reagents, kits 1 mL Not Available 09 075 887 001 9 075 887 001 09075887001 9075887001 09075887001 KAPA Probes Resuspension Buffer, 5 ml KAPA Probes Resuspension Buffer 07613336198131 Reagents, kits 5 mL Not Available 09 075 852 001 9 075 852 001 09075852001 9075852001 09075852001 KAPA Universal Enhancing Oligos 96 rxn KAPA Universal Enhancing Oligos 07613336198155 Reagents, kits 96 reactions Not Available 09 718 729 001 9 718 729 001 09718729001 9718729001 09718729001 KAPA HyperExome V2 Probes, 1152 rxn KAPA HyperExome V2 Probes, 1152 rxn 07613336216033 Reagents, kits 1,152 reactions Not Available 09 718 672 001 9 718 672 001 09718672001 9718672001 09718672001 KAPA HyperExome V2 Probes, 192 rxn KAPA HyperExome V2 Probes, 192 rxn 07613336216002 Reagents, kits 192 reactions Not Available 09 075 836 001 9 075 836 001 09075836001 9075836001 09075836001 KAPA Universal Enhancing Oligos 24 rxn KAPA Universal Enhancing Oligos 07613336198162 Reagents, kits 24 reactions Not Available 09 718 702 001 9 718 702 001 09718702001 9718702001 09718702001 KAPA HyperExome V2 Probes, 768 rxn KAPA HyperExome V2 Probes, 768 rxn 07613336216026 Reagents, kits 768 reactions Not Available 09 718 664 001 9 718 664 001 09718664001 9718664001 09718664001 KAPA HyperExome V2 Probes, 96 rxn KAPA HyperExome V2 Probes, 96 rxn 07613336215999 Reagents, kits 96 reactions Not Available 10 655 534 001 10655534001 KAPA HyperCapture Reagent V2 Kit, 384rxn KAPA HyperCapture Reagent V2 Kit, 384rxn Other Services 384 reactions Not Available 09 075 828 001 9 075 828 001 09075828001 9075828001 09075828001 KAPA HyperCapture Reagent Kit, 96 rxn KAPA HyperCapture Reagent kit 07613336198179 Reagents, kits 96 reactions Not Available 10 620 145 001 10620145001 KAPA HyperCapture Reagent V2 Kit, 24 rxn KAPA HyperCapture Reagent V2 Kit, 24 rxn 07613336242315 Reagents, kits 24 reactions Not Available 10 655 577 001 10655577001 KAPA HyperExome V2 EvoPrep Boost,192smpl KAPA HyperExome V2 EvoPrep Boost,192smpl Other Services 192 samples Not Available 09 075 879 001 9 075 879 001 09075879001 9075879001 09075879001 KAPA Probes Resuspension Buffer , 1 ml KAPA Probes Resuspension Buffer 07613336198148 Reagents, kits 1 mL Not Available 10 620 188 001 10620188001 KAPA HyperCapture Reagent V2 Kit, 96 rxn KAPA HyperCapture Reagent V2 Kit, 96 rxn 07613336242322 Reagents, kits 96 reactions Not Available 09 075 780 001 9 075 780 001 09075780001 9075780001 09075780001 KAPA HyperCapture Bead Kit, 24 rxn KAPA HyperCapture Bead kit 07613336198209 Reagents, kits 24 reactions Not Available 10 655 542 001 10655542001 KAPA HyperExome V2 EvoPlus Boost,192smpl KAPA HyperExome V2 EvoPlus Boost,192smpl Other Services 192 samples Not Available 09 718 737 001 9 718 737 001 09718737001 9718737001 09718737001 KAPA HyperExome V2 Probes, 1536 rxn KAPA HyperExome V2 Probes, 1536 rxn 07613336216040 Reagents, kits 1,536 reactions Not Available 09 718 648 001 9 718 648 001 09718648001 9718648001 09718648001 KAPA HyperExome V2 Probes, 24 rxn KAPA HyperExome V2 Probes, 24 rxn 07613336215975 Reagents, kits 24 reactions Not Available 09 075 798 001 9 075 798 001 09075798001 9075798001 09075798001 KAPA HyperCapture Bead Kit, 96 rxn KAPA HyperCapture Bead kit 07613336198193 Reagents, kits 96 reactions Not Available 09 718 699 001 9 718 699 001 09718699001 9718699001 09718699001 KAPA HyperExome V2 Probes, 384 rxn KAPA HyperExome V2 Probes, 384 rxn 07613336216019 Reagents, kits 384 reactions Not Available 09 718 656 001 9 718 656 001 09718656001 9718656001 09718656001 KAPA HyperExome V2 Probes, 48 rxn KAPA HyperExome V2 Probes, 48 rxn 07613336215982 Reagents, kits 48 reactions Not Available 09 718 630 001 9 718 630 001 09718630001 9718630001 09718630001 KAPA HyperExome V2 Probes, 12 rxn KAPA HyperExome V2 Probes, 12 rxn 07613336215968 Reagents, kits 12 reactions Not Available

KAPA HyperExome V2 Probes and Kits

Target enrichment probes for exome capture

RUO

Expertly designed probes that effectively capture challenging genomic regions

The KAPA HyperExome V2 Probes are Roche’s brand new Whole Exome Sequencing solution delivering superior coverage of the recent versions of ACMGv3.1, RefSeq, CCDS, ClinVar, Ensembl, and COSMIC genomic databases within a compact capture target of 43.2 Mb with low sequencing requirements. The new T2T (telomere-to- telomere) genome assembly was utilized as part of the design process to identify potentially problematic regions not apparent in the GRCh38 genome assembly.

This new version is extensively optimized using real-world samples in the streamlined KAPA HyperCap Workflow, including the KAPA EvoPrep and KAPA EvoPlus library prep chemistries, to efficiently cover hard-to-capture regions.

When automating using the AVENIO Edge System, you can focus on your clinical research based on a high-performing and reproducible walk-away whole-exome sequencing workflow from DNA to confident variant calling.

Benefits at a glance<sup>1</sup>

Benefits at a glance¹

Maximize sequencing coverage starting from a better exome design

Deliver higher quality results by covering more regions from the key genomic databases

Figure 1. Superior probe database coverage by the KAPA HyperExome V2 design compared to other vendors’ designs across important genomic databases (even up to 17% better).

Database data retrieved in Jan 2023, unpadded capture target used to compare across vendors.

Achieve higher uniformity across the entire %GC range

Exceptionally uniform coverage even through the extremes of the GC% spectrum
Eliminate GC bias and cover equally well low and high GC regions with an optimized design and uniform library amplification using the KAPA EvoPrep or KAPA EvoPlus V2 Kits

Figure 2: Exceptional uniformity of normalized capture coverage across the extremes of the GC% spectrum.

Vendor sample prep protocols were followed with 8-plex o/n hybridizations for vendor A (48 data points from 6 replicate captures of 16 coriell DNAs), 8-plex o/n hybridizations for vendor T (72 data points from 16 coriell and 24 blood extracted DNAs). For KAPA HyperExome V2 Probes, the KAPA HyperCap Evolved Workflow v5 was followed. A total of 32 libraries were prepared with the KAPA EvoPlus Boost Kit, starting from 100 ng of blood extracted DNA samples, and were hybridized by 2 x 16-plex captures for each kit workflow. Final libraries were sequenced on a NovaSeqTM 6000 System at 2 x 100 bp and 60 M high-quality reads were analyzed per library and proportionally to the capture target for the other vendor exomes.

Exceptional content coverage

Cover your bases, see what others may find difficult to see
Add more value and confidence in your results with superior database regions’ coverage
Eliminate “blind” spots - Leave fewer unknowns

Figure 3: Percent bases covered by at least 30x across some of the key genomic databases such as Ensembl, RefSeq, and CCDS, based on a January 2024 snapshot.

KAPA HyperExome V2 Probes deliver better database coverage compared to vendors I, A, and T that are leaving more “blind” spots, which may lead to repeats or need for spike-in probes. Vendor sample prep protocols were followed with singleplex 4h hybridizations for vendor I (3 replicates, NA12878), 8-plex o/n hybridizations for vendor A (48 data points from 6 replicate captures of 16 coriell DNAs), 8- plex o/n hybridizations for vendor T (72 data points from 16 coriell and 24 blood extracted DNAs). For KAPA HyperExome V2 Probes the KAPA HyperCap Evolved Workflow v5 was followed. A total of 40 libraries were prepared with the KAPA EvoPrep Boost Kit and 40 libraries with the KAPA EvoPlus Boost Kit starting from 100 ng of blood extracted DNA samples (in 2 x 16-plex hybridizations) and from 100 ng of NA12878 DNA samples (in 4 x 1-plex hybridization and 4 replicates from an 8-plex hybridization). KAPA HyperExome V2 Probes enriched libraries were sequenced on a NovaSeqTM 6000 System at 2 x 100 bp and 60 M high-quality reads were analyzed per library. Vendor-enriched libraries were sequenced proportionally to their capture target size.¹

Walkaway automation for high performance by the AVENIO Edge System

Exceptional sequencing efficiency by the KAPA HyperExome V2 Probes with the AVENIO Edge System
Reproducible performance with reagents powered by KAPA
Streamlined whole-exome sequencing solution with the AVENIO Edge true walk-away automation

Figure 4: High sequencing efficiency delivered by the KAPA HyperExome V2 Probes when used on the AVENIO Edge System, delivering low PCR duplication (mean 1.76%), with high specificity (mean 80.5% reads on-target) and high uniformity (mean 1.46 Fold-80 base penalty).

Library Preparation and Target Enrichment based on 8-plex pre-capture pooling (n=24, NA12878 100 ng input in the AVENIO Edge HyperPrep Kit) – 60 M high-quality reads 2 x 150bp analyzed from a NovaSeqTM 6000 System.

Streamline targeted sequencing with the KAPA HyperCap Workflow

KAPA HyperCap Workflow delivers uniform libraries by combining the high conversion rate of KAPA HyperPrep, KAPA HyperPlus, KAPA EvoPrep, and KAPA EvoPlus Kits with KAPA HyperCap probes (such as KAPA HyperExome V2 Probes), creating a streamlined, single-vendor supported workflow.

Multiplex up to 16 samples in the same capture, and potentially post-capture multiplex more samples in the same sequencing lane, with KAPA UDI Primer Mixes,1-384
Reduce workflow complexity and hands-on time with KAPA Universal Enhancing Oligos, eliminating the need for adapter-matched blocking oligos
Automate the entire KAPA HyperCap Workflow without the need for a SpeedVac—now with all hybridization and bead wash steps at 55ºC

KAPA HyperExome V2 Probes and Kits specifications

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Product specifications	Value
Compatible platform	All Illumina sequencing instruments
Library type	DNA
Starting material	Genomic DNA, cfDNA
Input amount	0.1 ng - 500 ng

Automation-friendly NGS library preparation

Thinking of automating library preparation? Roche provides NGS automation support with a menu of automated methods for KAPA library prep reagents on non-Roche liquid handling vendors, and on Roche's AVENIO Edge System.

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Fully automated whole-exome sequencing (WES) on the AVENIO Edge System

No prior NGS and/or automation experience required
As little as 20 minutes of deck setup time for the entire WES workflow
Automated quantification, normalization, and pooling
Complementary support team to help you along the way

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hg38 design files for the KAPA HyperExome V2 Probes

The KAPA HyperExome V2 panel covers coding exon sequences from RefSeq (06/29/2022), ClinVar (06/29/2022), and Ensembl release v106. It includes 529 sample tracking SNPs and a set of 96 probes. The total design size is 43.2 Mb, with design files provided in hg38 and converted hg19 genome assemblies.

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Do you have questions about our products or services? We’re here to help. Contact a Roche representative in your region.

KAPA products are for Research Use Only. Not for use in diagnostic procedures.

The AVENIO Edge System is a Class 1 US IVD and a Class A CE IVD (under IVDR) and is intended for downstream diagnostic applications. For the EU, Roche is collaborating with Platomics to enable laboratories to help automate document generation on Platomics’ multi-stakeholder platform for workflows incorporating the AVENIO Edge System. Reagents and kits mentioned are for Research Use Only. They are not intended for diagnostic procedures. The AVENIO Edge System can be used for Research Use Only (RUO) workflows.

References

F. Hoffmann-La Roche. Data on file.

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KAPA HyperExome V2 Probes and Kits

Expertly designed probes that effectively capture challenging genomic regions

Benefits at a glance¹

KAPA HyperExome V2 Probes and Kits specifications