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Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Membrane Others CD34 IVD Tonsil Placenta CD34 (QBEnd/10) Mouse Monoclonal Antibody PID00000064 Mouse QBEnd/10 05267323001 CELL MARQUE, CD34 CD34 00841683100089 Reagents, kits 50 test dispenser Not Available undefined CD34 (QBEnd/10) Mouse Monoclonal Primary Antibody is intended for laboratory use in the detection of the CD34 protein in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use. en CD34 (QBEnd/10) Mouse Monoclonal Antibody (this antibody) may be used as the primary
antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue
sections. In general, immunohistochemical staining allows the visualization of antigens
via the sequential application of a specific antibody (primary antibody) to the antigen, a
secondary antibody (link antibody) to the primary antibody, an enzyme complex and a
chromogenic substrate with interposed washing steps. The enzymatic activation of the
chromogen results in a visible reaction product at the antigen site. The specimen may then be
counterstained and a coverslip applied. Results are interpreted using a light microscope and
aid in the differential diagnosis of pathophysiological processes, which may or may not be
associated with a particular antigen.
This antibody is optimally diluted to be compatible with VENTANA detection kits and
BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for
recommended staining protocols. Each step in the staining protocol includes incubation for
a precise time at a specific temperature. At the end of each incubation step, the sections are
rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound
material that would hinder the desired reaction in subsequent steps. To minimize evaporation
of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied
in the slide stainer. For more detailed information on instrument operation, refer to the
appropriate BenchMark IHC/ISH instruments Operator’s Manual. en