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Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Cytoplasm Others Cytokeratin 35betaH11 IVD Prostate Cytokeratin (35betaH11) Mouse Monoclonal Antibody RTD000819 Mouse 35betaH11 05267471001 CELL MARQUE, CK,35 BETAH11 Cytokeratin (35betaH11) 00841683100126 Reagents, kits 50 tests Not Available true Cytokeratin (35betaH11) Mouse Monoclonal Primary Antibody is intended for laboratory use in the detection of the cytokeratin 8 and 18 proteins in formalin-fixed, paraffin-embedded tissue stained on VENTANA BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use. en Cytokeratin (35betaH11) Mouse Monoclonal Antibody (this antibody) may be used as the
primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded
tissue sections. In general, immunohistochemical staining allows the visualization of antigens
via the sequential application of a specific antibody (primary antibody) to the antigen, a
secondary antibody (link antibody) to the primary antibody, an enzyme complex and a
chromogenic substrate with interposed washing steps. The enzymatic activation of the
chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and
aid in the differential diagnosis of pathophysiological processes, which may or may not be
associated with a particular antigen.
This antibody is optimally diluted to be compatible with VENTANA detection kits and
BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for
recommended staining protocols. Each step in the staining protocol includes incubation for
a precise time at a specific temperature. At the end of each incubation step, the sections are
rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound
material that would hinder the desired reaction in subsequent steps. To minimize evaporation
of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied
in the slide stainer. For more detailed information on instrument operation, refer to the
appropriate BenchMark IHC/ISH instruments Operator’s Manual. en