Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Cytoplasm Others IgM polyclonal IVD Lymph Node Tonsil IgM Rabbit Polyclonal Antibody RTD000962 Rabbit 05267641001 CELL MARQUE, IGM IgM (polyclonal) 00841683100201 Reagents, kits 50 tests Not Available true IgM Rabbit Polyclonal Primary Antibody is intended for laboratory use in the detection of IgM in formalin-fixed, paraffin-embedded tissue stained on VENTANA BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use. en IgM Rabbit Polyclonal Antibody (this antibody) may be used as the primary antibody forimmunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. Ingeneral, immunohistochemical staining allows the visualization of antigens via the sequentialapplication of a specific antibody (primary antibody) to the antigen, a secondary antibody(link antibody) to the primary antibody, an enzyme complex and a chromogenic substratewith interposed washing steps. The enzymatic activation of the chromogen results in a visiblereaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits andBenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section forrecommended staining protocols. Each step in the staining protocol includes incubation fora precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unboundmaterial that would hinder the desired reaction in subsequent steps. To minimize evaporationof the aqueous reagents from the specimen-containing slide, a coverslip solution is appliedin the slide stainer. For more detailed information on instrument operation, refer to theappropriate BenchMark IHC/ISH instruments Operator’s Manual. en