For in vitro diagnostic use. Others OptiView Amplification Kit IVD OptiView Amplification Kit RTD001076 06718663001 OptiView Amplification Kit (250) OptiView Amplification Kit 04015630984749 Reagents, kits 860-099 250 tests Not Available true 06396518001 OptiView Amplification Kit OptiView Amplification Kit 04015630983834 Reagents, kits 760-099 50 tests Not Available true The OptiView Amplification Kit may be used with the OptiView DAB IHC Detection Kit (Cat. No. 760-700 / 06396500001), rabbit and mouse primary antibodies, and BenchMark IHC/ISH instruments to achieve preferred immunohistochemistry (IHC) staining. The OptiView Amplification Kit includes an HQ hapten conjugate (OptiView Amplifier), corresponding substrate (OptiView Amplification H2O2), and mouse anti-HQ monoclonal antibody containing HRP (OptiView Amplification Multimer).When the amplification option is selected in the OptiView DAB IHC Detection Kit protocol, following the deposition of the OptiView HRP Multimer reagent, the OptiView Amplifier and OptiView Amplification H2O2 are added to the slide. After an incubation step the slide is washed and OptiView Amplification Multimer is added and incubated. Following a wash step the OptiView DAB and OptiView H2O2 is added to the slide followed by the OptiView Copper reagent. The bound specific antibodies to antigens in the tissue are visualized by a brown colored precipitate. en OptiView Amplification Kit may be used in conjunction with the OptiView DAB IHC Detection Kit to increase the staining intensity of mouse and rabbit primary antibodies. The kit is to be used for qualitative staining of formalin-fixed, paraffin-embedded tissue, or frozen tissue on a BenchMark IHC/ISH instrument with VENTANA primary antibodies and ancillary reagents, visualized by light microscopy.The clinical interpretation of any staining, or the absence of staining, must be complemented by morphological studies and evaluation of proper controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.This product is intended for in vitro diagnostic (IVD) use. en