Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Nucleus Others SATB2 EP281 IVD Colon Adenocarcinoma Colon SATB2 (EP281) Rabbit Monoclonal Antibody RTD001252 Rabbit EP281 08313415001 SATB2 (EP281) PAb, Cell Marque SATB2 (EP281) 00841683143659 Reagents, kits 50 Tests Not Available undefined SATB2 (EP281) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the detection of the SATB2 protein in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use. en SATB2 (EP281) Rabbit Monoclonal Primary Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fi xed, paraffi n-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specifi c antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the diff erential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specifi c temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual. en