For in vitro diagnostic use. Others Special Stains Van Gieson CS IVD Special Stains Van Gieson CS PID00000053 860-073 Special Stains Van Gieson CS 07613336178867 50 tests per kit true Special Stains Van Gieson CS 09185992001 Not Available Reagents, kits en Special Stains Van Gieson CS used in conjunction with Elastic Stain Core Kit is intended for laboratory use in staining non-target tissue components and providing contrast with target stains in sections of formalin-fixed, paraffin-embedded (FFPE) tissue stained on a BenchMark Special Stains instrument. This reagent is intended as a counterstain in special stains applications. This product is intended for in vitro diagnostic (IVD) use.  en Verhoeff’s Van Gieson method is a regressive stain. Dye binding occurs mainly via Van der Waals forces and hydrogen bonds. Verhoeff’s elastic stain binds to elastic fibers with higher affinity than compared to other cell structures. The Ferric Chloride Oxidizer oxidizes the Hematoxylin to hematein. Hematein is a compound that has high affinity for iron together with the second mordant, Lugol’s Iodine, to form an iron hematein complex and chelates with the elastic fibers in the tissue. The Differentiator uses lower strength of ferric chloride. This step is used to differentiate the target stain from non-specific binding. The rinsing of the Differentiator removes the loosely bound stain from cell structures other than elastic fibers.The Special Stains Van Gieson CS stains red blood cells and muscle yellow and collagen pink to provide contrast with the dark purple to black elastic fibers.This kit is optimized for use on BenchMark Special Stains instruments. The reagents are applied to tissue on microscope slides and mixed over the entire specimen.