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"Chapters": [
{
"Name": "Storage Conditions (Product)",
"Value": "The Alcian Blue for PAS should be stored at 15-30 °C.",
"Language": "en",
"Country": "XG",
"Code": "Storage Conditions (Product)"
},
{
"Name": "Content",
"Value": "The reagent vial is supplied in a barcode labeled carrier to insert into the reagent tray of the instrument.
Each kit contains sufficient reagent for 75 tests:
One 22 mL vial of Alcian Blue contains approximately 1.2% alcian blue in a 3% acetic acid solution.
One vial insert with sipping straw.",
"Language": "en",
"Country": "XG",
"Code": "Content"
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"Name": "Intended Use",
"Value": "Alcian Blue for PAS, in conjunction with the PAS Staining Kit, is intended for laboratory use as a qualitative histologic stain to differentiate between acid and neutral mucins by light microscopy in sections of formalin-fixed, paraffin- embedded (FFPE) tissue on a BenchMark Special Stains instrument.
This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
This product is intended for in vitro diagnostic (IVD) use.",
"Language": "en",
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"Name": "Background Information",
"Value": "Alcian Blue for PAS is a single bottle kit used in conjunction with the PAS Staining Kit. The PAS Staining Kit is a modification of a technique originally described by McManus in 1946 to visualize mucins, glycogen, basement membrane and fungal organisms through the combination of oxidation of polysaccharides by periodic acid and staining with the Schiff’s reagent.1
Mucins are a key component of goblet cells, intestinal mucosal epithelial cells, whose function is to synthesize and secrete mucus.2 Goblet cells are normally found in the epithelium of the small and large intestines, and are generally absent from the esophagus or stomach.3 Alcian blue staining in conjunction with Periodic acid-Schiff (PAS) can differentiate pseudogoblet cells from true goblet cells.2 Originally described by McMannus in 1946, PAS is a staining technique to visualize mucins through the combination of oxidation of polysaccharides by periodic acid and staining with the Schiff reagent.4
Alcian Blue for PAS is used to aid the pathologist in the identification of goblet cells. The presence of goblet cells in the esophagus and stomach is abnormal and the stain aids the pathologist in the diagnosis of intestinal metaplasia and Barrett’s esophagus.
1. Layton C, Bancroft JD. Bancroft’s Theory and Practice of Histological Techniques. In: Elsevier; 2019. Accessed 02/15/2021.
2.Dao D, Le PH. Histology, Goblet Cells. In 04/09/2020 ed. Treasure Island, FL: StatPearls; 2020.
3. Pleskow D, Tolga E. Barrett’s Esophagus: Emerging Evidence for Improved Clinical Practice. Elsevier Inc.; 2016.
4. Carson F, Hladik C. Histotechnology: A Self Instructional Text, 3rd edition. Hong Kong: American Society for Clinical Pathology Press; 2009. ",
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"Name": "Principle",
"Value": "The PAS Staining Kit uses Periodic Acid reagent to oxidize glycols to aldehydes. The Schiff’s Reagent forms a colorless dialdehyde compound that is transformed to the magenta staining of glycol containing cellular components.4 The chemical properties of Alcian Blue enable the dye to detect weakly acidic mucins in goblet cells. Alcian Blue for PAS, with a pH of 2.5, stains acid mucopolysaccharides blue.5 This kit is optimized for use on BenchMark Special Stains instruments. The reagent is applied to tissue on microscope slides and mixed over the entire specimen.
4. Carson F, Hladik C. Histotechnology: A Self Instructional Text, 3rd edition. Hong Kong: American Society for Clinical Pathology Press; 2009.
5. Fagan C, Dapson RW, Horobin RW, Kiernan JA. Revised tests and standards for Biological Stain Commission certification of Alcian blue dyes. Biotech Histochem. 2020;95(5):333-340.",
"Language": "en",
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This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
This product is intended for in vitro diagnostic (IVD) use.",
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