For in vitro diagnostic use. Others Alcian Blue Staining Kit IVD Colon Small Intestine Alcian Blue Staining Kit RTD000640 860-002 Alcian Blue Staining Kit 04015630971367 75 tests true ALC BLUE STAINING KIT PH2.5 05279186001 Not Available Reagents, kits en The Alcian Blue Staining Kit is intended for laboratory use as a qualitative histologic stain to demonstrate weakly acidic mucopolysaccharides by light microscopy in sections of formalin- fixed, paraffin-embedded (FFPE) tissue stained on a BenchMark Special Stains instrument.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This product is intended for in vitro diagnostic (IVD) use. en The staining reaction is based on the reaction of Alcian Blue with a pH of 2.5 and polyanionic compounds. Alcian Blue is a polyvalent, basic, water soluble dye that derives its blue color from the copper phthalocyanine group which is modified with cationic solubilizing agents.3,5 During this reaction, salt linkages are formed between Alcian Blue and the acid groups of the acid mucopolysaccharides, staining them blue. The Nuclear Fast Red Counterstain is applied to provide a pink contrasting background.This kit is optimized for use on BenchMark Special Stains instruments. The reagents are applied to tissue on microscope slides and mixed over the entire specimen.3. Scott JE. Alcian dyes: I.C.I. cease manufacture and release details of composition. Histochemie. 1973;37(4):379-380.5. Fagan C, Dapson RW, Horobin RW, Kiernan JA. Revised tests and standards for Biological Stain Commission certification of Alcian blue dyes. Biotech Histochem. 2020;95(5):333-340.