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AXELIOS 1 Core Library Prep Kit
RUO
For Research Use Only. Not for use in diagnostic procedures.
Library prep reagents for both DNA and cDNA (from RNA), as well as mechanical and enzymatic fragmentation inputs
The AXELIOS 1 DNA Library Prep Kits serve as the foundation for the entire workflow, accommodating diverse sample inputs, including both DNA and cDNA derived from RNA. Designed for versatility, these kits support both mechanical and enzymatic fragmentation methods to suit your specific experimental needs. By integrating proven KAPA technology, this kit delivers exceptional molecule conversion and recovery rates, ensuring that high-quality, representative libraries are generated at the very first step to provide the best possible input for downstream synthesis.1
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Overview
Product specifications
View full tableProduct specifications
Sample Type |
Method |
Sequencing readout |
Target input range |
Human genomic DNA |
WGS
|
Duplex |
25-500 ng |
Cell-free DNA |
Duplex |
2-10 ng |
|
FFPE DNA |
Duplex |
100-300 ng (dependent on quality) |
|
Human genomic DNA |
Rapid WGS |
Duplex |
2000 ng |
RNA |
WTS |
Simplex |
25-150 ng |
AXELIOS 1 Core Library Kit contents (24 reactions)
View full tableAXELIOS 1 Core Library Kit contents (24 reactions)
Cap or plate colour |
Component name |
Red |
AXELIOS 1 FragTail ReadyMix |
Violet |
AXELIOS 1 ERAT Enzyme Mix |
Violet |
AXELIOS 1 ERAT Buffer |
Yellow |
AXELIOS 1 Ligation Enzyme |
Yellow |
AXELIOS 1 Ligation Buffer |
Orange |
AXELIOS 1 Ligation Digest |
Kit box dimensions (width x length x height): 50mm x 75mm x 55mm
Technical documents
Access important product documentation including relevant certificates and other resources.
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For Research Use Only. Not for use in diagnostic procedures. AXELIOS is a trademark of Roche.
References
- F. Hoffmann-La Roche Ltd. Data on file.
- Chen C, et al. Single-Cell Whole Genome Analyses by Linear Amplification via Transposon Insertion (LIANTI). Science. 2017; 356(6334): 189–194.
- Jia H, et al. Chasing Sequencing Perfection: Marching Toward Higher Accuracy and Lower Costs. Genomics, Proteomics & Bioinformatics. 2024; 22(2): qzae024.