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"Name": "Storage Conditions (Product)",
"Value": "Upon receipt and when not in use, store at 2-8°C. Do not freeze.",
"Language": "en",
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1, is a B-cell-specific coactivator which has been mapped to chromosome 11q23.
1 Expression of BOB.1/OBF.
1 is restricted largely to mature B-cells, with germinal center B-cells normally staining for BOB.
1.2,3 Analyses of BOB.1/OBF
1 expression in a variety of established B-cell lines representing different stages of B-cell development has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immunoreactive for BOB.
1 Conversely, only some cases of classical Hodgkin lymphoma show BOB.
1 immunoreactivity within the Hodgkin and Reed-Sternberg cells.
3-7 Expression of BOB.1/OBF.
1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.
8-12 B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
11-121. Junker S, et al. Assignment of the human gene for Oct-Binding Factor-1 (OBF1), a B-Cell-spefific coactivator of Octamer-Binding transcription Factors1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization. Genomics. 1996; 33:143-5.
2. Dabbs DJ. Diagnostic Immunohistochemistry, Third Edition. Saunders. 2006.
3. Steimle-Grauer SA, et al. Expression patterns of transcription factors in progressively transformed germinal centers and hodgkin lymphoma. Virchows Arch. 2003; 442:284-93.
4. Valsami S, et al. A clinicopathological study of B-cell differentiation markers and transcription factors in classical hodgkin lymphoma: a potential prognostic role of mum1/ IRF4. Haematologica. 2007; 92:1343-50.
5. Stein H, et al. Down regulation of Bob.1 /OBF.1 and oct2 in classical Hodgkins disease but no in lymphocyte perdominant Hodgkins disease correlates with immunglobulin transcription. Blood. 2001; 97:496-501.
6. Hertel CB, et al. Loss of B cell identity correlates with loss of B Cell-specific transcription factors in Hodgkin / Reed-Sternberg Cells of classical Hodgkin lymphoma. Oncogene. 2002; 21:4908-20.
7. Pileri SA, et al. Primary mediastinal B_cell lymphoma: high frequence of BCL-6 mutations and consistent expression of the transcription factors OCT-2, Bob.1 , PU.1 in the absence of immunoglobulins. Am J Pathology 2003; 162:243-53.
8. Hoefnagel JJ, et al. Expression of B-cell transcription factors in primary cutaneous B-cell lymphoma. Moder Pathology. 2006; 19:1270-6.
9. Greiner A, et al. Up-regulation of BOB.1/OBF.1 expression in normal germinal center B cells and germinal center-derived lymphomas. Am J Pathol. 2000;156:501-7.
10. Kuroda H, et al. Primary diffuse large B-cell lymphoma of the breast. Breast Cancer. 2007; 14:317-22.
11. Sáez AI, et al. Analysis of octamer-binding transcription factors Oct2 and Oct1 and their coactivator BOB.1/OBF.1 in lymphomas. Mod Pathol. 2002; 15:211-20.
12. Loddenkemper C, et al. Differential Emu enhancer activity and expression of BOB.1/OBF.1, Oct2, PU.1, and immunoglobulin in reactive B-cell populations, B-cell non-Hodgkin lymphomas, and Hodgkin lymphomas. J Pathol. 2004; 202:60-9.",
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This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specifi c temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.",
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