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"Immunohistochemistry (IHC)", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Upon receipt and when not in use, store at 2-8°C. Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "BOB.1 (SP92) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the detection of BOB.1 in formalin-fixed, paraffin-embedded tissue stained on VENTANA BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "Oct-binding factor-1 (OBF1), also known as BOB¹, is a B-cell-specific coactivator which has been mapped to chromosome 11q23.¹ Expression of BOB.1/OBF.¹ is restricted largely to mature B-cells, with germinal center B-cells normally staining for BOB.^1.2,3 Analyses of BOB.1/OBF¹ expression in a variety of established B-cell lines representing different stages of B-cell development has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immunoreactive for BOB.¹ Conversely, only some cases of classical Hodgkin lymphoma show BOB.¹ immunoreactivity within the Hodgkin and Reed-Sternberg cells.^3-7 Expression of BOB.1/OBF.¹ has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.^8-12 B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.^11-12

1. Junker S, et al. Assignment of the human gene for Oct-Binding Factor-1 (OBF1), a B-Cell-spefific coactivator of Octamer-Binding transcription Factors1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization. Genomics. 1996; 33:143-5.
2. Dabbs DJ. Diagnostic Immunohistochemistry, Third Edition. Saunders. 2006.
3. Steimle-Grauer SA, et al. Expression patterns of transcription factors in progressively transformed germinal centers and hodgkin lymphoma. Virchows Arch. 2003; 442:284-93.
4. Valsami S, et al. A clinicopathological study of B-cell differentiation markers and transcription factors in classical hodgkin lymphoma: a potential prognostic role of mum1/ IRF4. Haematologica. 2007; 92:1343-50.
5. Stein H, et al. Down regulation of Bob.1 /OBF.1 and oct2 in classical Hodgkins disease but no in lymphocyte perdominant Hodgkins disease correlates with immunglobulin transcription. Blood. 2001; 97:496-501.
6. Hertel CB, et al. Loss of B cell identity correlates with loss of B Cell-specific transcription factors in Hodgkin / Reed-Sternberg Cells of classical Hodgkin lymphoma. Oncogene. 2002; 21:4908-20.
7. Pileri SA, et al. Primary mediastinal B_cell lymphoma: high frequence of BCL-6 mutations and consistent expression of the transcription factors OCT-2, Bob.1 , PU.1 in the absence of immunoglobulins. Am J Pathology 2003; 162:243-53.
8. Hoefnagel JJ, et al. Expression of B-cell transcription factors in primary cutaneous B-cell lymphoma. Moder Pathology. 2006; 19:1270-6.
9. Greiner A, et al. Up-regulation of BOB.1/OBF.1 expression in normal germinal center B cells and germinal center-derived lymphomas. Am J Pathol. 2000;156:501-7.
10. Kuroda H, et al. Primary diffuse large B-cell lymphoma of the breast. Breast Cancer. 2007; 14:317-22.
11. Sáez AI, et al. Analysis of octamer-binding transcription factors Oct2 and Oct1 and their coactivator BOB.1/OBF.1 in lymphomas. Mod Pathol. 2002; 15:211-20.
12. Loddenkemper C, et al. Differential Emu enhancer activity and expression of BOB.1/OBF.1, Oct2, PU.1, and immunoglobulin in reactive B-cell populations, B-cell non-Hodgkin lymphomas, and Hodgkin lymphomas. J Pathol. 2004; 202:60-9.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Principle", "Value": "BOB.1 (SP92) Rabbit Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specifi c temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "BOB.1 (SP92) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the detection of BOB.1 in formalin-fixed, paraffin-embedded tissue stained on VENTANA BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }