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"Value": "Anti-Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody is intended for laboratory use in the qualitative immunohistochemical detection of cytokeratin 5 by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.
This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
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One 5 mL dispenser of anti-Cytokeratin 5/6 (D5/16B4) antibody contains approximately 52 μg of a mouse monoclonal antibody.
The antibody is diluted in a phosphate buffered saline containing carrier protein and 0.10% ProClin 300, a preservative.
Specific antibody concentration is approximately 10.4 μg/mL. There is no known nonspecific antibody reactivity observed in this product.
Anti-Cytokeratin 5/6 (D5/16B4) antibody is a monoclonal antibody produced as cell culture supernatant.
Refer to the appropriate VENTANA detection kit method sheet for detailed descriptions of: Principle of the Procedure, Material and Methods, Specimen Collection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Results, and Limitations.",
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"Value": "Anti-Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody (anti-Cytokeratin 5/6 (D5/16B4) antibody) is a mouse monoclonal antibody raised for the detection of cytokeratin (CK) 5. CK5 is a type II CK (high molecular weight, 62 kDa) expressed in the basal and myoepithelial cells of various epithelia and glandular structures including the bronchi, prostate and breast.1,2
CK5 is expressed in the basal epithelium of the lung and its overexpression is an indicator of malignant squamous differentiation in pulmonary lesions.1,3 Malignant squamous differentiation is characteristic of the squamous cell carcinoma (SCCA) subtype of non-small cell lung cancer (NSCLC).4,5 The immunohistochemistry (IHC)-based detection of CK5 with anti-Cytokeratin 5/6 (D5/16B4) antibody can be used to aid in the distinction between pulmonary SCCA and pulmonary adenocarcinoma (ADC).
In addition, CK5 is expressed in mesothelial cells of the pulmonary pleura and is overexpressed in epithelioid malignant mesothelioma (MM).6 CK5 is consistently absent in pulmonary ADC that confounds the diagnosis of epithelioid MM.6,7 Thus, detection of CK5 with anti-Cytokeratin 5/6 (D5/16B4) antibody can be used to aid in the differential diagnosis of epithelioid MM from lung ADC.
CK5 is expressed in prostatic basal cells and the loss of CK5 expression is indicative of neoplasms that have breached the basal membrane.1,8 The detection of CK5 in basal cells is a feature of normal and benign prostatic processes and the absence of CK5 is indicative of prostate adenocarcinoma.9,10 Thus, detection of CK5 in prostatic basal cells with anti-Cytokeratin 5/6 (D5/16B4) antibody may be used to aid in the differentiation of benign and malignant prostate lesions.
Additionally, CK5 is expressed in myoepithelial cells (MECs) in normal breast tissue and benign processes.11,12 The absence of MECs is a diagnostic feature of invasive processes.13-15 In cases where the MEC layer is difficult to evaluate via histology alone, ancillary IHC assays for MEC markers can be used.13-15 Several MEC markers exist and recommendations include use of IHC studies for at least two markers to demonstrate the presence or absence of myoepithelial cells.13-15 The detection of CK5 in breast MECs with anti-Cytokeratin 5/6 (D5/16B4) antibody may be used to aid in distinguishing non-invasive from invasive breast neoplasms.
The antibody clone D5/16B4, raised for the detection of CK5, is also reactive to CK6, another type II CK (60 kDa) that shares 86% sequence homology to CK5. CK6 is expressed in hyperproliferative keratinocytes and is not used as a basal cell marker.1 The antibody D5/16B4 is often referred to as CK5/6 antibody in the pathology literature.
This antibody may be used as part of a panel of IHC studies.
1. Moll R, Divo M, Langbein L. The Human Keratins: Biology and Pathology. Histochem Cell Biol. 2008;129(6):705-733.
2. Purkis PE, Steel JB, Mackenzie IC, et al. Antibody Markers of Basal Cells in Complex Epithelia. J Sci. 1990;97 ( Pt 1):39-50
3. Blobel GA, Moll R, Franke WW, et al. Cytokeratins in normal lung and lung carcinomas. I. Adenocarcinomas, squamous cell carcinomas and cultured cell lines. Virchows Archiv B, Cell Pathology Including Molecular Pathology. 1984;45(4):407- 429.
4. Kriegsmann K, Cremer M, Zgorzelski C,et al. Agreement of Ck5/6, P40, and P63 Immunoreactivity in Non-Small Cell Lung Cancer. Pathology. 2019
5. Warth A, Muley T, Herpel E, et al. Large-Scale Comparative Analyses of Immunomarkers for Diagnostic Subtyping of Non-Small-Cell Lung Cancer Biopsies. Histopathology. 2012;61(6):1017-1025.
6. Moll R, Dhouailly D, Sun TT. Expression ofKeratin 5 as a Distinctive Feature of Epithelial and Biphasic Mesotheliomas. An Immunohistochemical Study Using Monoclonal Antibody Ae14. Virchows Archiv B, Cell Pathol Incl Mol Pathol. 1989;58(2):129-145.
7. Galateau-Salle F, Churg A, Roggli V, et al. The 2015 World Health Organization Classification of Tumors of the Pleura:Advances since the 2004 Classification. J Thorac Oncol. 2016;11(2):142-154.
8. Yang Y, Hao J, Liu X, et al. Differential expression of cytokeratin mRNA and protein in normal prostate, prostatic intraepithelial neoplasia, and invasive carcinoma. Am J Pathol. 1997;150(2):693-704.
9. Abrahams NA, Ormsby AH, Brainard J. Validation of cytokeratin 5/6 as an effective substitute for keratin 903 in the differentiation of benign from malignant glands in prostate needle biopsies. Histopathology. 2002;41(1):35-41
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"Language": "en",
"Country": "XG",
"Code": "Background Information"
},
{
"Name": "Principle",
"Value": "The anti-Cytokeratin 5/6 (D5/16B4) antibody binds cytokeratin 5 and cytokeratin 6 in formalin-fixed, paraffin-embedded (FFPE) tissue sections and exhibits a cytoplasmic staining pattern. This antibody can be visualized using ultraView Universal DAB Detection Kit (Cat. No. 760-500 / 05269806001). Refer to the respective method sheet for further information.",
"Language": "en",
"Country": "XG",
"Code": "Principle"
},
{
"Name": "Product Purpose",
"Value": "Anti-Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody is intended for laboratory use in the qualitative immunohistochemical detection of cytokeratin 5 by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.
This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
This antibody is intended for in vitro diagnostic (IVD) use.",
"Language": "en",
"Country": "XG",
"Code": "Product Purpose"
}
]
}
}
]
}
