For in vitro diagnostic use. Others Elastic Stain Core Kit IVD Elastic Stain Core Kit PID00000052 09 185 984 001 9 185 984 001 09185984001 9185984001 09185984001 Elastic Stain Core Kit Elastic Stain Core Kit 07613336178850 Reagents, kits 860-047 50 tests per kit Not Available true Elastic Stain Core Kit, used in conjunction with Special Stains Van Gieson CS is intended for laboratory use as a qualitative histologic stain to demonstrate elastic fibers by light microscopy in sections of formalin-fixed, paraffin-embedded (FFPE) tissue stained on a BenchMark Special Stains instrument.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This product is intended for in vitro diagnostic (IVD) use. en Verhoeff’s Van Gieson method is a regressive stain. Dye binding occurs mainly via Van der Waals forces and hydrogen bonds. Verhoeff’s elastic stain binds to elastic fibers withhigher affinity than compared to other cell structures. The Ferric Chloride Oxidizer oxidizes the Hematoxylin to hematein. Hematein is a compound that has high affinity for iron together with the second mordant, Lugol’s Iodine, to form an iron hematein complex and chelates with the elastic fibers in the tissue. The Differentiator uses lower strength ferric chloride. This step is used to differentiate the target stain from non-specific binding. The rinsing of the Differentiator removes the loosely bound stain from cell structures other than elastic fibers.The Special Stains Van Gieson CS stains red blood cells and muscle yellow and collagen pink to provide contrast with the dark purple to black elastic fibers.This kit is optimized for use on BenchMark Special Stains instruments. The reagents are applied to tissue on microscope slides and mixed over the entire specimen. en

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