{ "ProductData": { "ID": "RTD000935", "ProductType": "Others", "BrandName": "Green for Trichrome", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use.", "DisclaimerGroup1": "IVD", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ "Formalin-Fixed Paraffin-Embedded Tissue (FFPET)" ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "Colon", "Kidney", "Liver", "Skin" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Green for Trichrome" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Product_RTD000935_im_en.jpg", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "05-0025", "StructureNodeStatus": "Active", "NodeName": "Special Stains Staining Kits" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "120-00", "StructureNodeStatus": "Active", "NodeName": "Special Stains" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "080-00", "StructureNodeStatus": "Active", "NodeName": "Pathology Lab" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "010", "StructureNodeStatus": "Active", "NodeName": "Histopathology" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "Active", "NodeName": "NOT APPLICABLE" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "35-000-00", "StructureNodeStatus": "Active", "NodeName": "Special Stain" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "06521916001", "MaterialDescription": "GREEN FOR TRICHROME", "RegisteredProductName": "Green for Trichrome", "GTIN": "04015630984381", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "860-032", "PackSizePIM360": "75 tests", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "", "ReadyForUse": "true", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "INS_3198", "BrandName": "BenchMark Special Stains", "ProductNameAddition": "", "ReferenceType": "Instrument", "Classification": [ { "IdentifierofStructureSystem": "1", "NameofStructureSystem": "GPCH", "StructureNodeID": "7682", "StructureGroupPath": "Histology->Systems / Digital Products->Primary Staining->BenchMark Special Stains", "StructureGroupName": "BenchMark Special Stains", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "10-000-00", "StructureGroupPath": "Analyzer Instruments and Systems", "StructureGroupName": "Analyzer Instruments and Systems", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "080", "StructureGroupPath": "BenchMark", "StructureGroupName": "BenchMark", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "080-00", "StructureGroupPath": "Pathology Lab", "StructureGroupName": "Pathology Lab", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "010", "StructureGroupPath": "Histopathology", "StructureGroupName": "Histopathology", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "120-00", "StructureGroupPath": "Special Stains", "StructureGroupName": "Special Stains", "StructureNodeStatus": "Active" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "The Green for Trichrome should be stored at 2-8°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Intended Use", "Value": "Green for Trichrome, in conjunction with Trichrome Staining Kit, is intended for laboratory use as a qualitative histologic stain to demonstrate collagen fibers, muscle and connective tissue by light microscopy in sections of formalin-fixed, paraffin-embedded (FFPE) tissue stained on a BenchMark Special Stains instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This product is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Content", "Value": "The reagent vials are supplied in barcode labeled carriers to insert into the reagent tray of the instrument. Each kit contains sufficient reagent for 75 tests:

One 27 mL vial of Trichrome Green reagent contains approximately 1% fast green and 0.75% hydrochloric acid.

One vial insert with sipping straw.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Background Information", "Value": "Green for Trichrome is a single bottle kit which is used in conjunction with the Trichrome Staining Kit. Trichrome Staining Kit is a modification of Masson’s Trichrome Stain.¹ Fibrosis occurs when normal tissue architecture and function are compromised due to the presence of excess extracellular matrix components.² Fibrosis can occur in several tissues/organs and can lead to permanent scarring and organ malfunction.³ The primary marker of fibrosis is overgrowth and/or scarring which is associated with collagen volume.⁴ The trichrome staining method employs three dyes that distinctly color smooth muscle, collagen, and connective tissues.⁵ This staining allows for a more accurate assessment of collagen in a tissue specimen by enhancing contrast of these tissue components.

Green for Trichrome, in conjunction with the Trichrome Staining Kit, is used to aid the pathologist in the assessment of fibrosis.⁶

References

1. Lillie RD. Further experiments with the Masson Trichrome modification of Mallory’s connective tissue stain. Stain Technol. 1940:15;82.
2. Lupher ML, Gallatin WM. Regulation of Fibrosis by the Immune System. In:2006:245-288.
3. Kuter DJ, Bain B, Mufti G, et al. Bone Marrow Fibrosis: Pathophysiology and Clinical Significance of Increased Bone Marrow Stromal Fibres. Br J Haematol. 2007;139(3):351-362.
4. Wynn TA. Cellular and Molecular Mechanisms of Fibrosis. J Pathol. 2008;214(2):199-210.
5. Bancroft JD, Layton C. Connective and Other Mesenchymal Tissues with Their Stains. In: Bancroft's Theory and Practice of Histological Techniques.2019:153-175.
6. Tretheway D, Jain A, LaPoint R, et al. Should Trichrome Stain Be Used on All Post-Liver Transplant Biopsies with Hepatitis C Virus Infection to Estimate the Fibrosis Score? Liver Transpl. 2008;14(5):695-700.
7. Carson F, Hladik C. Histotechnology: A Self Instructional Text, 3rd edition. Hong Kong: American Society for Clinical Pathology Press; 2009.
8. Clinical and Laboratory Standards Institute (CLSI). CLSI Web site. http://www.clsi.org/. Accessed November 3, 2011.
9. Occupational Safety and Health Standards: Occupational exposure to hazardous chemicals in laboratories. (29 CFR Part 1910.1450). Fed. Register.
10. Directive 2000/54/EC of the European Parliament and Council of 18 September 2000 on the protection of workers from risks related to exposure to biological agents at work.

", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Principle", "Value": "Trichrome Bouin’s is applied which acts as a mordant to allow penetration of subsequent dyes. Nuclei are stained with Trichrome Hematoxylin A and Trichrome Hematoxylin B (forms a complex of iron hematoxylin). Cytoplasm and muscle is stained with Trichrome Red, containing Biebrich scarlet and acid fuchsin. Trichrome Mordant removes the excess red from the collagen which is stained with Green for Trichrome, which contains fast green.

This kit is optimized for use on BenchMark Special Stains instruments. The reagents are applied to tissue on microscope slides and mixed over the entire specimen.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "Green for Trichrome, in conjunction with Trichrome Staining Kit, is intended for laboratory use as a qualitative histologic stain to demonstrate collagen fibers, muscle and connective tissue by light microscopy in sections of formalin-fixed, paraffin-embedded (FFPE) tissue stained on a BenchMark Special Stains instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This product is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }