For in vitro diagnostic use. Others Hematoxylin IVD Hematoxylin RTD000938 05266726001 HEMATOXYLIN Hematoxylin 04015630971169 Reagents, kits 760-2021 250 tests Not Available true Hematoxylin is a modified Gill's hematoxylin intended for laboratory use in staining cellular nuclei on slides containing cells from frozen tissue, formalin-fixed, paraffin-embedded tissue, or cytologic preparations on a BenchMark IHC/ISH instrument. This reagent is intended as a counterstain to immunohistochemistry and immunocytochemistry applications.This reagent is intended for in vitro diagnostic use. en In general, immunohistochemistry (IHC) or immunocytochemistry (ICC) staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. At the end of the incubation step, the instrument washes the slides to stop the reaction and remove unbound material. The specimen may then be counterstained with Hematoxylin solution, which is applied to the slide and mixed over the entire specimen area. Hematoxylin stains nuclei blue through the binding of a mordant dye complex to nucleic acids and histone proteins of the heterochromatin. The specimen may then be post counterstained and coverslip applied. en