For general laboratory use. Others ISH Amplification Kit GLU ISH Amplification Kit RTD000975 07729022001 ISH AMPLIFICATION KIT ISH Amplification Kit 04015630967179 Reagents, kits 760-300 50 tests Not Available true The ISH Amplification Kit may be used in conjunction with DNP detection to improve the signal intensity of DNP-labeled probes. The kit is to be used for qualitative staining by in situ hybridization (ISH) in sections of formalin-fixed, paraffin-embedded tissue on VENTANA IHC/ISH instruments with VENTANA ISH probes and ancillary reagents.The result is visualized by light microscopy. en The ISH Amplification Kit may be used with DNP detection, VENTANA ISH probes, and VENTANA IHC/ISH instruments to achieve preferred ISH staining. The ISH Amplification Kit includes an HQ hapten conjugate (ISH Amplifier), corresponding substrate (ISH Amplification H2O2), and mouse anti-HQ monoclonal antibody containing HRP (ISH Amplification Multimer).When the amplification option is selected in the ISH staining protocol, following the deposition of the DNP detection kit HRP multimer reagent, the ISH Amplifier and ISH Amplification H2O2 are added to the slide. After an incubation step the slide is washed and ISH Amplification Multimer is added and incubated. Following a wash step the remaining DNP detection kit reagents are added to the slide. The bound specific probes to nucleic acid target in the tissue are visualized by a black colored precipitate. en