For in vitro diagnostic use. Others ISH Protease 3 IVD ISH Protease 3 RTD001008 05273331001 ISH PROTEASE 3 ISH Protease 3 04015630971886 Reagents, kits 780-4149 200 tests Not Available true ISH Protease 3 is intended for enzymatic digestion of formalin-fixed, paraffin-embedded tissue sections, or whole cell preparations on a BenchMark IHC/ISH instrument. This reagent removes protein that surrounds the target nucleic acid sequences of interest during in situ hybridization applications.This reagent is intended for in vitro diagnostic (IVD) use. en ISH Protease 3 is used with probes, accessory reagents and the BenchMark IHC/ISH instrument to achieve appropriate in situ hybridization (ISH) staining.Enzymatic digestion using ISH Protease 3 removes cross-linking of proteins to ensure that the probe of interest reaches its target DNA or RNA sequence.1 ISH Protease 3 is a low activity enzyme formulation that cleaves proteins in the formalin-fixed, paraffin-embedded (FFPE) tissue section, exposing the target nucleic acid for hybridization by the probe.Digestion is followed by probe application and is used in combination with VENTANA detection kits. ISH Protease 3 may be used for target nucleic acid sequences requiring light digestion for optimal staining.1. Beesley JE. Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences. Boston: Birkhäuser, 2001. en