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"StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "Inactive" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "The Jones Light Green Staining Kit should be stored at 2-8°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Background Information", "Value": "Jones Light Green Staining Kit is a modification of Jones Methenamine Silver procedure.¹ The glomerulus has a glomerular capillary basement membrane between it and the urinary space which is then encapsulated by the Bowman’s capsule.^2,3 Glomerular disease occurs when there is a disruption to the glomerular filtration barrier that may allow red blood cells or plasma proteins to pass through the barrier.⁴

The Jones Light Green Staining Kit can highlight extracellular matrix proteins, adhesions to the Bowman’s capsule, and breaks within the capillary basement membrane. This stain provides contrast and resolution of these delicate structures. ^5,6,7 The methenamine-silver complex in this stain is used to demonstrate the carbohydrate components of basement membranes.⁵

The Jones Light Green Staining Kit demonstrates capillary basement membranes to aid the pathologist in the diagnosis of glomerular disease in kidney tissue.

References

1. Koski JP. Silver methenamine‑borate (MB): Cost reduction with technical improvement in silver nitrate‑gold chloride impregnations. J Histotechnol. 1981;4:115.
2. Kitching AR, Hutton HL. The Players: Cells Involved in Glomerular Disease. Clin J Am Soc Nephrol. 2016;11(9):1664-1674.
3. Mac-Moune Lai F, Szeto CC, Choi PC, et al. Isolate Diffuse Thickening of Glomerular Capillary Basement Membrane: A Renal Lesion in Prediabetes? Mod Pathol. 2004;17(12):1506-1512.
4. Hebert LA, Parikh S, Prosek J, et al. Differential Diagnosis of Glomerular Disease: A Systematic and Inclusive Approach. Am J Nephrol. 2013;38(3):253-266.
5. Bancroft JD, Layton C. Connective and Other Mesenchymal Tissues with Their Stains. In: Bancroft's Theory and Practice of Histological Techniques.2019:153-175.
6. Cathro HP, Shen SS, Truong LD. Diagnostic Histochemistry in Medical Diseases of the Kidney. Semin Diagn Pathol. 2018;35(6):360-369.
7. Herrera GA, Turbat-Herrera EA. Renal Diseases with Organized Deposits: An Algorithmic Approach to Classification and Clinicopathologic Diagnosis. Arch Pathol Lab Med. 2010;134(4):512-531.
8. Jones DB. Nephrotic Glomerulonephritis. Am J Pathol. 1957;33(2):313-329.
9. Carson F, Hladik C. Histotechnology: A Self Instructional Text, 3rd edition. Hong Kong: American Society for Clinical Pathology Press; 2009.
10. Occupational Safety and Health Standards: Occupational exposure to hazardous chemicals in laboratories. (29 CFR Part 1910.1450). Fed. Register.
11. Directive 2000/54/EC of the European Parliament and Council of 18 September 2000 on the protection of workers from risks related to exposure to biological agents at work.

", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Intended Use", "Value": "The Jones Light Green Staining Kit is intended for laboratory use as a qualitative histologic stain to demonstrate capillary basement membranes by light microscopy in sections of formalin-fixed, paraffinembedded (FFPE) tissue stained on the BenchMark Special Stains instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This product is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Content", "Value": "The reagent vials are supplied in barcode labeled carriers to insert into the reagent tray of the instrument. Each kit contains sufficient reagent for 40 tests:

One 19 mL vial of Jones Periodic Acid contains approximately 1% periodic acid.

One 19 mL vial of Jones Silver A contains approximately 1% silver nitrate.

One 15 mL vial of Jones Silver B contains approximately 2% sodium borate and approximately 14% methenamine.

One 15 mL vial of Toner contains approximately 1% gold chloride.

One 15 mL vial of Fixer contains approximately 2% sodium thiosulfate.

One 19 mL vial of Jones Hematoxylin contains modified Mayer’s Hematoxylin (contains sodium iodate and ethylene glycol).

One 10 mL vial of Jones Light Green contains approximately 1% Light Green Y in acetic acid.

Seven vial inserts with sipping straws.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "The staining reaction is based on aldehyde reduction of silver ions to metallic silver under alkaline conditions. Jones Periodic Acid is used to oxidize carbohydrates to aldehyde groups. The combined Jones Silver A and Jones Silver B solutions form a methenaminesilver complex that is easily reduced to metallic silver by the aldehyde groups. To enhance staining contrast, toning of tissue specimen is completed using gold chloride within the Toner reagent. Fixer, with thiosulfate, stops the reaction and removes any unreduced silver from the section.^5,8 Nuclei are stained with Jones Hematoxylin and Jones Light Green is applied to provide a contrasting background.

This kit is optimized for use on BenchMark Special Stains instruments. The reagents are applied to tissue on microscope slides and mixed over the entire specimen.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "The Jones Light Green Staining Kit is intended for laboratory use as a qualitative histologic stain to demonstrate capillary basement membranes by light microscopy in sections of formalin-fixed, paraffinembedded (FFPE) tissue stained on the BenchMark Special Stains instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This product is intended for in vitro diagnostic (IVD) use.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }