For Research Use Only. Not for use in diagnostic procedures. Others LightMix Digital EGFR Ex19del RUO LightMix® Digital EGFR_Ex19del 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000572 Kit with reagents for a maximum of 32 (HiSense plate) or 46 (Universal plate) digital PCR reactionsTIB MOLBIOL order number: 20-3001-32 10068469001 LightMix Digital EGFR_Ex19del LightMix Digital EGFR_Ex19del 04260159335170 Reagents, kits for a maximum of 32 (HiSense plate) or 46 (Universal plate) digital PCR reactions Not Available false Using PCR methodology, an 87 bp fragment (wild-type sequence) covering the EGFR exon 19 deletions is amplified with specific primers. The mutation site is analyzed using two labeled probes: a reference probe (HEX-labeled) and an indel probe (FAM-labeled). The reference probe is designed to bind to the amplicon irrespective of mutation presence, and the indel probe is designed to bind to the wild-type sequence but not to any of the mutated sequences. The PC included in the kit contains 4-6 % synthetic mutant target in wild-type genomic DNA and is equivalent to ~17 ng/µl when dissolved in 60 µl of PCR grade water. It is recommended to always include at least one PC and a No- Template-Control (NTC) (e.g. PCR grade water) in each run. en The assay has been experimentally verified to a sensitivity of 0.05 % at 1 copies per partition (cpp) with an artificial target. With real samples the sensitivity may differ. en