For in vitro diagnostic use. Others LightMix in-vitro diagnostics kit ACE I D IVD LightMix® in-vitro diagnostics kit ACE I/D 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. TIBMOLBIOL-0182 Detection of the Insertion / Deletion DNA variation in the ACE gene for use with the Roche Diagnostics LightCycler® Instruments 07 161 182 001 7 161 182 001 07161182001 7161182001 07161182001 LightMix ACE I/D LightMix ACE I/D 04260159332452 Reagents, kits 40-0637-64 64 reactions Not Available false This kit allows the detection of the Angiotensin I converting enzyme (ACE) insertion / deletion polymorphism (ACE I/D) in genomic human DNA obtained from a nucleic acid extract made from peripheral blood.The deletion allele (D) is associated with a number of disease risks, in particular myocardial infarction (for more information described in the method sheet see 3.1 Medical Background).This product is intended to help the clinician to analyze the genetic background for patients with an increased ACE serum level which do not respond to ACE inhibitors, patients who had a myocardial infarct, individuals with a left ventricular hypertrophy, individuals with the Angiotensin Receptor II A166C mutation.The kit is not intended to be the only basis for therapy decision. The patient´s mutation status should be considered alongside other disease factors.Note: The performance of the assay can be guaranteed only when used with LightCycler® Instruments. en Using PCR methodology, 480 bp (I) or 193 bp (D) fragment of the ACE gene is amplified with specific primers. The PCR fragment is analyzed using an internally labeled SimpleProbe® oligomer binding to the region spanning the mutation site. During the melting curve analysis the temperature is slowly increased. The probe leaves at a specific temperature (Tm) causing a fluorescence decrease. Any mismatch covered by the probe destabilizes the hybrid and lowers the Tm.In this product the probe matches the sequence of the deleted genotype and the presence of the insertion will result in a reduced Tm.Reading of the genotype results is based on the melting temperatures compared to the supplied standards. If permitted by the instrument software reading of the genotype results can be achieved by the automated genotyping module (instrument-dependent: software module 'Melt Curve Genotyping').Automated genotyping results must be reviewed by eye for deviating curves and intermediate melting point temperatures. In case that automated typing fails to report consistent genotype results, the genotype must be deducted from the melting temperatures following the criteria described in the method sheet, chapter 7. en Analytical SpecificityThe specificity to the target gene and the suitability of the PCR amplification employed in the present test was demonstrated by sequencing of the amplicon.Analytical Sensitivity Detection of serial dilutions of several heterozygous human genomic DNAs has revealed that the limit of detection of the present kit is 250 copies (1.5 ng).Diagnostic Specificity and Sensitivity A total number of 62 different genomic DNA samples from individuals of Caucasian origin were analyzed in parallel with a previously established kit. Study results: Results for both analytical methods were in 100% concordance. In particular, 22 samples were homozygous for the genotype D/D, 36 samples were heterozygous (genotype D/I) and 4 samples were homozygous for the genotype I/I. en

LightMix^® in-vitro diagnostics kit ACE I/D

Detection of the Insertion / Deletion DNA variation in the ACE gene for use with the Roche Diagnostics LightCycler^® Instruments

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LightMix^® in-vitro diagnostics kit ACE I/D

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