For in vitro diagnostic use. Others LightMix in-vitro diagnostics kit Factor II G20210A CE-IVD IVD LightMix® in-vitro diagnostics kit Factor II G20210A CE-IVD 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. TIBMOLBIOL-0123 Detection of the G20210A DNA variation in the Factor II (prothrombin) genefor use with the Roche Diagnostics LightCycler® Instruments 06 896 502 001 6 896 502 001 06896502001 6896502001 06896502001 LightMix Factor II Prothrombin LightMix Factor II Prothrombin 04260159332162 Reagents, kits 40-0593-64 Reagents for 64 reactions Not Available false This diagnostic PCR kit allows the detection of the Factor II (F2, prothrombin, OMIM 176930) G20210A mutation (NCBI dbSNP: rs1799963) from genomic human DNA extracted from peripheral blood. This F2 mutation belongs, like Activated Protein C (APC) resistance (Factor V Leiden), or deficiency of Antithrombin III, Protein C or Protein S, to the group of heredity risk factors for thrombosis. This product is indicated to help the clinician to analyze the genetic back-ground of patients showing : venous thrombosis pulmonary embolism premature ischemic stroke premature myocardial infarction in women family history for the factor II mutation family history of stroke, pulmonary embolism, deep vein thrombosis in first degree relatives under 50. As the risks for thrombosis increases for those individuals bearing Factor II and Factor V mutations, patients should be analyzed also for the Factor V (Leiden) polymorphism G1691A, for example using the LightMix® Kit 40-0594 (Roche SAP No.: 06896529001). The kit is not intended to be the only basis for therapy decision. The patient´s mutation status should be considered alongside other disease factors. Note: The performance of the assay can be guaranteed only when used with LightCycler® Instruments (see 1.3.2 for details). en Using PCR methodology, a 106 bp fragment of the Factor II gene is amplified with specific primers. The PCR fragment is analyzed using an internally labeled SimpleProbe® oligomer binding to the region spanning the mutation site. During melting curve analysis, the temperature is slowly increased. The probe leaves at a specific temperature (Tm) causing a fluorescence decrease. Any mismatch covered by the probe destabilizes the hybrid and lowers the Tm. In this product the probe matches the sequence of the wild type genotype and the presence of a mutation variant will result in a reduced Tm. Reading of the genotype results is based on the melting temperatures compared to the supplied standards. If permitted by the instrument software, reading the genotype results can be achieved by the automated genotyping module (instrument-dependent: software module 'Melt Curve Genotyping') or by the kit specific LightCycler® Analysis Package (LCAP) for LightCycler® PRO Instrument. Automated genotyping results must be reviewed manually for deviating curves and intermediate melting point temperatures. In case that automated typing fails to report consistent genotype results, the genotype must be deduced from the melting temperatures following the criteria described in chapter 7. en

LightMix^® in-vitro diagnostics kit Factor II G20210A CE-IVD

Detection of the G20210A DNA variation in the Factor II (prothrombin) gene
for use with the Roche Diagnostics LightCycler^® Instruments

IVD

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LightMix^® in-vitro diagnostics kit Factor II G20210A CE-IVD

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