For in vitro diagnostic use. Others LightMix in-vitro diagnostics kit MTHFR C677T CE-IVD IVD LightMix® in-vitro diagnostics kit MTHFR C677T CE-IVD 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. TIBMOLBIOL-0119 Detection of the C677T DNA variation in the MTHFR genefor use with the Roche Diagnostics LightCycler® Instruments 06 896 367 001 6 896 367 001 06896367001 6896367001 06896367001 LightMix MTHFR C677T LightMix MTHFR C677T 04260159330441 Reagents, kits 40-0129-64 Reagents for 64 reactions Not Available false This kit allows the detection of the Methylenetetrahydrofolate Reductase (MTHFR, OMIM: 607093) C677T single nucleotide polymorphism rs1801133 in genomic human DNA from a nucleic acid extract obtained from peripheral blood. The C677T gene mutation encodes an protein variant exhibiting a decreased enzymatic activity. Homozygous carriers of the gene 677 T/T variant, and to less extend also compound heterozygous individuals with the allele combination C677T and A1298C, may exhibit elevated homocysteine plasma levels, resulting in a higher risk to develop vascular disease, thrombosis, and hypertension. This product is intended to help the clinician to analyze the genetic background showing increased homocysteine levels or a propensity to thrombosis. The present test can be performed in addition or after a phenotypical assay of homocysteine levels. C677T heterozygous patients should also be analyzed for the second prevalent MTHFR polymorphism A1298C, for example using the LightMix® Kit 40-0269. The kit is not intended to be the only basis for therapy decision. The patient´s mutation status should be considered alongside other disease factors. Note: The performance of the assay can be guaranteed only when used with LightCycler® Instruments (see 1.3.2 for details). en Using PCR methodology, a 288 bp fragment of the MTHFR gene is amplified with specific primers. The PCR fragment is analyzed using an internally labeled SimpleProbe® oligomer binding to the region spanning the mutation site.During the melting curve analysis the temperature is slowly increased. The probe leaves at a specific temperature (Tm) causing a fluorescence decrease. Any mismatch covered by the probe destabilizes the hybrid and lowers the Tm.In this product the probe matches the sequence of the wild type genotype and the presence of a mutation will yield a reduced Tm.Reading of the genotype results is based on the melting temperatures compared to the supplied standards. If permitted by the instrument software reading of the genotype results can be achieved by the automated genotyping module (instrument-dependent: software module 'Melt Curve Genotyping').Automated genotyping results must be reviewed by eye for deviating curves and intermediate melting point temperatures. In case that automated typing fails to report consistent genotype results, the genotype must be deduced from the melting temperatures following the criteria described in the method sheet, chapter 7. en

LightMix^® in-vitro diagnostics kit MTHFR C677T CE-IVD

Detection of the C677T DNA variation in the MTHFR gene
for use with the Roche Diagnostics LightCycler^® Instruments

IVD

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LightMix^® in-vitro diagnostics kit MTHFR C677T CE-IVD

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