For in vitro diagnostic use. Others LightMix in-vitro diagnostics kit U. urealyticum U. parvum CE-IVD IVD LightMix® in-vitro diagnostics kit Ureaplasma urealyticum / Ureaplasma parvum CE-IVD 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000677 Detection of Ureaplasma urealyticum and Ureaplasma parvum genomic DNAfor use with the Roche Diagnostics LightCycler® Instruments 07 821 034 001 7 821 034 001 07821034001 7821034001 07821034001 LightMix Ureaplasma urealyticum/parvum LightMix Ureaplasma urealyticum/parvum 04260159333794 Reagents, kits 40-0461-32 96 reactions Not Available false This device is an in-vitro nucleic acid amplification test for the detection and discrimi- nation of genomic DNA from Ureaplasma urealyticum (U.u.) and Ureaplasma parvum (U.p.) from nucleic acid extracts obtained from human urogenital swabs. The present diagnostic device must be used by qualified personnel only. Note: The performance of the assay can be guaranteed only when used with LightCycler® Instruments (see 1.2.2 for details). en This device amplifies and detects fragments from the genomes of Ureaplasma from a nucleic acid extract obtained from swabs.A partialy conserved fragment of the urease gene is amplified with specific primers (182 bp long) and detected with a common fluorescine probe, a specific probe labeled with the dye LightCycler® Red 640 for U. parvum or a specific probe labeled with the dye LightCycler® Red 690 for U. urealyticum.Pathogens are discriminated by dye colors.After every PCR cycle the probes bind during the annealing step to the PCR gener- ated target and bring the fluorophores in close proximity. The donor fluorophore fluo- rescein is excited by light, part of the energy is transferred to the acceptor dye and detected. The fluorescence intensities are plotted against the cycle number, giving a picture of the amplification and calculating a crossing point (Cp) which is related to the log of the concentration of target in the sample.Hybridization Probes can be used to identify the PCR product in a melting curve anal- ysis in order to verify that the right product has been formed. The melting point (Tm) is dependent on length and G+C content, but also upon the degree of homology to the sensor probe. In this device the Tm is used for discrimination; slight temperature shifts are acceptable as the shape of curve depends also on the amount of product.Any significant decrease in the melting temperature is an indication of a sequence variation, explaining an eventual lower signal level in the quantification plot and may give cause for a subsequent analysis of the sample or PCR product by DNA sequenc- ing (feel free to contact us at: [email protected] for assistance).The supplied control DNAs allow comparison with unknown patient samples. en

LightMix^® in-vitro diagnostics kit Ureaplasma urealyticum / Ureaplasma parvum CE-IVD

Detection of Ureaplasma urealyticum and Ureaplasma parvum genomic DNA
for use with the Roche Diagnostics LightCycler^® Instruments

IVD

User profile

Select your user profile

LightMix^® in-vitro diagnostics kit Ureaplasma urealyticum / Ureaplasma parvum CE-IVD

Overview

Ordering information

Technical documents

Access important product documentation including relevant certificates and other resources.

Compatible products

Related products

Contact us