For in vitro diagnostic use. Others LightMix in-vitro diagnostics kit VKORC1 IVD LightMix® in-vitro diagnostics kit VKORC1 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. TIBMOLBIOL-0253 Detection of the C1173T DNA variation in the VKORC1 genefor use with the Roche Diagnostics LightCycler® Instruments 07 805 985 001 7 805 985 001 07805985001 7805985001 07805985001 LightMix VKORC1 LightMix VKORC1 04260159332100 Reagents, kits 40-0302-64 64 reactions Not Available false This kit allows the detection of the common single-base 1173 C>T polymorphism in the Vitamin K epoxide reductase complex subunit 1 gene (VKORC1) in genomic human DNA from a nucleic acid extract obtained from peripheral blood, which is in 100% linkage with the VKORC1 –1639 G>A (rs9923231) promoter variant. Warfarin dosage may be adjusted based on Cytochrome 2C9 gene variants, and second line on VKORC1 gene variants. The following table is adapted from [1] : VKORC1 1173 CYP 2C9 *1/*1 *1/*2 *1/*3 *2/*2 *2/*3 *3/*3 CC 5-7 mg 5-7 mg 3-4 mg 3-4 mg 3-4 mg 0.5-2 mg CT 5-7 mg 3-4 mg 3-4 mg 3-4 mg 0.5-2 mg 0.5-2 mg TT 3-4 mg 3-4 mg 0.5-2 mg 0.5-2 mg 0.5-2 mg 0.5-2 mg This kit should be used in conjunction with CYP 2C9 gene testing and is not intended to be the only basis for a therapy decision. The ranges of doses are derived from different studies. Other factors, such as age, race, body weight, sex, concomitant medications, and comorbidities have to be accounted. Consider also more rare mutations which might have an influence on the Warfarin activity. Note: The performance of the assay can be guaranteed only when used with LightCycler® Instruments. en Using PCR methodology, a 176 bp fragment of the VKORC1 gene is amplified with specific primers. The fragment is detected with a mutation-specific detection probe internally labeled with SimpleProbe® 519 reagent.SimpleProbe® probes are fluorescent only when bound to complement DNA.The probe binds to a part of the amplified fragment spanning the mutation site.Any mismatch covered by the probe destabilizes the hybrid. During the melting curve analysis the temperature is slowly increased. The probe melts off at a specific melting temperature causing the fluorescence to decrease.In this product the probe matches the sequence of the mutated 1173T variant and the presence of the WildType C1173 genotype will result a reduced Tm.Reading of the genotype results is based on the melting temperatures compared to the supplied standards. If permitted by the instrument software reading of the genotype results can be achieved by the automated genotyping (instrument- dependent: software module 'Melt Curve Genotyping').Automated genotyping results must be reviewed by eye for deviating curves and intermediate melting point temperatures. In case that automated typing fails to report consistent genotype results, the genotype must be deducted from the melting temperatures following the criteria described in the method sheet, chapter 7.The supplied control DNA standards allow a comparison with clinical samples. en

LightMix^® in-vitro diagnostics kit VKORC1

Detection of the C1173T DNA variation in the VKORC1 gene
for use with the Roche Diagnostics LightCycler^® Instruments

IVD

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LightMix^® in-vitro diagnostics kit VKORC1

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