For life science research only. Not for use in diagnostic procedures. Others LightMix Kit BRAF V600E K LSR LightMix® Kit BRAF V600E/K 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. TIBMOLBIOL-0090 Kit with reagents for the detection of BRAF 600 mutations in genomic DNA using Roche Diagnostics LightCycler® 1.x/ 2.0 / 480 / 480 II / cobas z 480 Analyzer. 06 296 793 001 6 296 793 001 06296793001 6296793001 06296793001 LightMix BRAF V600E LightMix BRAF V600E 04260159331646 Reagents, kits 40-0406-96 96 reactions Not Available false This kit detects BRAF V600 E and K mutations from human genomic DNA extracts. The kit includes two separate reactions, both using primers generating a 212 bp long fragment, which is tested in a melting curve analysis for the presence of mutations. For use on Roche LightCycler® systems. en PCR results are obtained within 1.5 hours (cycling and melting curve) with the LightCycler® 2.0 Instrument and within 2 hours (cycling and melting curve) with the LightCycler® 480 Instrument.SensitivityThese reagents detect very low amounts of BRAF mutations DNA since the amplification of the wild type target DNA is suppressed by a competitor.The detection limit is dependent on the total amount of DNA and the respective mutation. With 100 ng total DNA the detecton limit of the MSR reaction for 600E is at least 1%, with 200 ng better than 0.5%. The detection limit for 600K with 10 - 500 ng is 0.5%. Increasing amounts of target DNA up to 500 ng per reaction increase the relative sensitivity.Table 1 en

LightMix^® Kit BRAF V600E/K

Kit with reagents for the detection of BRAF 600 mutations in genomic DNA using Roche Diagnostics LightCycler^® 1.x/ 2.0 / 480 / 480 II / cobas z 480 Analyzer.

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LightMix^® Kit BRAF V600E/K

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